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Gene/ORF C07H6.5 (cgh-1) Ace View of C07H6.5
C07H6.5 Details Help
Description  The ortholog of a RNA helicase ste13/ME31B/RCK/p54 that is required for oocyte and sperm function and the prevention of physiological germline-associated apoptosis; it is expressed in the early embryo and germline and localizes to P granules.
Homology Group (KOG)  ATP-dependent RNA helicase
GO Terms  gametogenesis (GO:0007276) ; anti-apoptosis (GO:0006916) ; mRNA metabolism (GO:0016071) ; cytoplasm (GO:0005737) ; RNA helicase activity (GO:0003724) ; ATP binding (GO:0005524) ; ATP dependent helicase activity (GO:0008026) ; nucleic acid binding (GO:0003676) ; helicase activity (GO:0004386)
Location   III:7495613..7498153
RNAi Phenotypes
Early_embryo Osmotic Integrity defective ; Osmotic Integrity defective
Postembryonic Adl
Larva Lva
Adult Reduced fecundity of injected worm ; Pvl ; Ste ; Ste ; Ste ; Rup ; No developing embryos seen 48 hours after dsRNA injection ; Stp ; Stp
External Links  wormbase.org NCBI.org NBrowse
Associated RNAi Experiments View Summary
Cenix:305-b5
Embryonic Phenotypes  Emb Penetrance None reported
Adult Phenotypes  Osmotic Integrity defective
RNAi Reagent Name & Type Cenix:305-b5 (PCR reagent)
Delivery Method  Injection
Remark  early embryonic: Osmotic Integrity
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
Cenix:341-g3
Embryonic Phenotypes  Emb Penetrance None reported
Adult Phenotypes  Osmotic Integrity defective
RNAi Reagent Name & Type Cenix:341-g3 (PCR reagent)
Delivery Method  Injection
Remark  early embryonic: Osmotic Integrity
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
JA:C07H6.5
Embryonic Phenotypes  Emb Penetrance 80 %
Adult Phenotypes  Pvl
Rup
Ste
Adult Phenotypes  Adl
RNAi Reagent Name & Type sjj_C07H6.5 (PCR reagent)
Laboratory  JA
Date  2003-1-10
Reference  Kamath RS et al. (2003) Nature "Systemic functional analysis of the Caenorhabditis elegans genome using ...."
PF:GL1_4D4
Embryonic Phenotypes  Emb Penetrance 80 - 100 %
Larval Phenotypes  Lva Observed in >=10% of progeny.
Adult Phenotypes  Ste
Stp Observed in >=10% of progeny.
No developing embryos seen 48 hours after dsRNA injection
Reduced fecundity of injected worm
RNAi Reagent Name & Type sjj_C07H6.5 (PCR reagent)
Delivery Method  Injection
Remark  PCR product contains flanking T7 promoter sequences
Laboratory  KK
Date  2002-11-19
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
Movies/Stacks/Images  /static/images/movie.gif
Simmer:C07H6.5
Adult Phenotypes  Stp
RNAi Reagent Name & Type sjj_C07H6.5 (PCR reagent)
Laboratory  NL
Date  2003-10-01
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
TH:305b5
Embryonic Phenotypes  Emb Penetrance None reported
RNAi Reagent Name & Type TH:305b5 (PCR reagent)
Remark  Phenotype comment -- Confirmed with independent dsRNA (C07H6.5 -RNA2; similar phenotype).
Laboratory  TH
Date  2000-11-16
Reference  Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...."
TH:341g3
Embryonic Phenotypes  Emb Penetrance None reported
RNAi Reagent Name & Type TH:341g3 (PCR reagent)
Remark  Phenotype comment -- Confirmed with independent dsRNA (C07H6.5 -RNA2; similar phenotype).
Laboratory  TH
Date  2000-11-16
Reference  Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...."
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