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Gene/ORF C14B9.4 (plk-1) Ace View of C14B9.4
C14B9.4 Details Help
Description  plk-1 encodes a serine/threonine polo-like kinase homologous to Drosophila polo and Saccharomyces cerevisiae CDC5; PLK-1 is required for meiotic nuclear envelope breakdown, polar body formation and extrusion, and proper chromosome segregation during meiosis, and additionally, may play a role in germline development; PLK-1 is proposed to phosphorylate REC-8, a meiotic-specific cohesin, thus promoting REC-8 degradation and subsequent separation of sister chromatids; PLK-1 is localized diffusely in the cytoplasm of mature oocytes, but during meiosis I becomes associated with pre-metaphase chromatin and then at anaphase with chromatin and the area between dividing chromatin; in embryos, PLK-1 localizes to centrosomes, metaphase chromatin, and during telophase, to the area between dividing chromatin.
GO Terms  regulation of cell cycle (GO:0000074) ; meiotic chromosome segregation (GO:0045132) ; polar body extrusion after meiotic divisions (GO:0040038) ; mitotic nuclear envelope breakdown (GO:0007077) ; centrosome (GO:0005813) ; protein serine/threonine kinase activity (GO:0004674) ; protein binding (GO:0005515) ; protein kinase activity (GO:0004672) ; ATP binding (GO:0005524) ; protein amino acid phosphorylation (GO:0006468)
Location   III:8100869..8104397
RNAi Phenotypes
Early_embryo Slow cell cycle ; Slow cell cycle ; Complex Phenotype ; Osmotic Integrity defective ; Catastrophic one-cell arrest ; Catastrophic one-cell arrest ; Osmotically/ pressure sensitive ; Osmotically/ pressure sensitive ; Multiple nuclei in 1, 2, or 4-cell embryos
Postembryonic Adl ; Adl
Larva Lvl ; Lvl
Adult Reduced fecundity of injected worm ; Reduced fecundity of injected worm ; Pvl ; Pvl ; Pvl ; Ste ; Ste ; Ste
External Links  wormbase.org NCBI.org NBrowse
Associated RNAi Experiments View Summary
Cenix:302-e10
Embryonic Phenotypes  Emb Penetrance None reported
Adult Phenotypes  Osmotic Integrity defective
RNAi Reagent Name & Type Cenix:302-e10 (PCR reagent)
Delivery Method  Injection
Remark  early embryonic: Osmotic Integrity
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
Cenix:302-f10
Embryonic Phenotypes  Emb Penetrance None reported
Adult Phenotypes  Complex Phenotype
RNAi Reagent Name & Type Cenix:302-f10 (PCR reagent)
Delivery Method  Injection
Remark  early embryonic: Complex Phenotype
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
JA:C14B9.4
Embryonic Phenotypes  Emb Penetrance 100 %
Larval Phenotypes  Lvl
Adult Phenotypes  Pvl
Adult Phenotypes  Adl
RNAi Reagent Name & Type sjj_C14B9.4 (PCR reagent)
Laboratory  JA
Date  2003-1-10
Reference  Kamath RS et al. (2003) Nature "Systemic functional analysis of the Caenorhabditis elegans genome using ...."
JA:K06H7.1
Embryonic Phenotypes  Emb Penetrance 100 %
Larval Phenotypes  Lvl
Adult Phenotypes  Pvl
Adult Phenotypes  Adl
RNAi Reagent Name & Type sjj_K06H7.1 (PCR reagent)
Laboratory  JA
Date  2003-1-10
Reference  Kamath RS et al. (2003) Nature "Systemic functional analysis of the Caenorhabditis elegans genome using ...."
PF:GL1_4E6
Embryonic Phenotypes  Emb Penetrance 100 %
Adult Phenotypes  Ste
Reduced fecundity of injected worm
Adult Phenotypes  Catastrophic one-cell arrest
Multiple nuclei in 1, 2, or 4-cell embryos
Osmotically/ pressure sensitive
Slow cell cycle
RNAi Reagent Name & Type sjj_K06H7.1 (PCR reagent)
Delivery Method  Injection
Remark  PCR product contains flanking T7 promoter sequences
Laboratory  KK
Date  2002-11-19
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
Movies/Stacks/Images  /static/images/movie.gif /static/images/movie.gif
PF:GL1_5C1
Embryonic Phenotypes  Emb Penetrance 100 %
Adult Phenotypes  Ste
Reduced fecundity of injected worm
Adult Phenotypes  Catastrophic one-cell arrest
Osmotically/ pressure sensitive
Slow cell cycle
RNAi Reagent Name & Type sjj_C14B9.4 (PCR reagent)
Delivery Method  Injection
Remark  PCR product contains flanking T7 promoter sequences
Laboratory  KK
Date  2002-11-19
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
Movies/Stacks/Images  /static/images/movie.gif /static/images/movie.gif
Simmer:C14B9.4
Embryonic Phenotypes  Emb Penetrance 100 %
RNAi Reagent Name & Type sjj_C14B9.4 (PCR reagent)
Laboratory  NL
Date  2003-10-01
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
Simmer:K06H7.1
Embryonic Phenotypes  Emb Penetrance 100 %
RNAi Reagent Name & Type sjj_K06H7.1 (PCR reagent)
Laboratory  NL
Date  2003-10-01
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
TH:302e10
Embryonic Phenotypes  Emb Penetrance None reported
RNAi Reagent Name & Type TH:302e10 (PCR reagent)
Remark  Phenotype comment -- Confirmed with independent dsRNA against K06H7.1 (K06H7.1-RNA1); see also Chase et al. (2000). A shorter dsRNA against K06H7.1, covering exons 3 and 4 (K06H7.1-RNA2), did not result in a phenotype, suggesting inaccurate gene prediction.
Laboratory  TH
Date  2000-11-16
Reference  Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...."
TH:302f10
Embryonic Phenotypes  Emb Penetrance None reported
RNAi Reagent Name & Type TH:302f10 (PCR reagent)
Remark  Phenotype comment -- Confirmed with independent dsRNA against K06H7.1 (K06H7.1-RNA1); see also Chase et al. (2000). A shorter dsRNA against K06H7.1, covering exons 3 and 4 (K06H7.1-RNA2), did not result in a phenotype, suggesting inaccurate gene prediction.
Laboratory  TH
Date  2000-11-16
Reference  Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...."
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