Ace View of C14B9.4 | |
C14B9.4 Details | |
Description | plk-1 encodes a serine/threonine polo-like kinase homologous to Drosophila polo and Saccharomyces cerevisiae CDC5; PLK-1 is required for meiotic nuclear envelope breakdown, polar body formation and extrusion, and proper chromosome segregation during meiosis, and additionally, may play a role in germline development; PLK-1 is proposed to phosphorylate REC-8, a meiotic-specific cohesin, thus promoting REC-8 degradation and subsequent separation of sister chromatids; PLK-1 is localized diffusely in the cytoplasm of mature oocytes, but during meiosis I becomes associated with pre-metaphase chromatin and then at anaphase with chromatin and the area between dividing chromatin; in embryos, PLK-1 localizes to centrosomes, metaphase chromatin, and during telophase, to the area between dividing chromatin. |
GO Terms | regulation of cell cycle (GO:0000074) ; meiotic chromosome segregation (GO:0045132) ; polar body extrusion after meiotic divisions (GO:0040038) ; mitotic nuclear envelope breakdown (GO:0007077) ; centrosome (GO:0005813) ; protein serine/threonine kinase activity (GO:0004674) ; protein binding (GO:0005515) ; protein kinase activity (GO:0004672) ; ATP binding (GO:0005524) ; protein amino acid phosphorylation (GO:0006468) |
Location | III:8100869..8104397 |
RNAi Phenotypes | |
External Links | |
Associated RNAi Experiments | |
Cenix:302-e10 | |
Embryonic Phenotypes | Emb Penetrance None reported |
Adult Phenotypes | Osmotic Integrity defective |
RNAi Reagent Name & Type | Cenix:302-e10 (PCR reagent) |
Delivery Method | Injection |
Remark | early embryonic: Osmotic Integrity |
Laboratory | TH |
Date | 2005-3-24 |
Reference | Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...." |
Cenix:302-f10 | |
Embryonic Phenotypes | Emb Penetrance None reported |
Adult Phenotypes | Complex Phenotype |
RNAi Reagent Name & Type | Cenix:302-f10 (PCR reagent) |
Delivery Method | Injection |
Remark | early embryonic: Complex Phenotype |
Laboratory | TH |
Date | 2005-3-24 |
Reference | Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...." |
JA:C14B9.4 | |
Embryonic Phenotypes | Emb Penetrance 100 % |
Larval Phenotypes | Lvl |
Adult Phenotypes | Pvl |
Adult Phenotypes | Adl |
RNAi Reagent Name & Type | sjj_C14B9.4 (PCR reagent) |
Laboratory | JA |
Date | 2003-1-10 |
Reference | Kamath RS et al. (2003) Nature "Systemic functional analysis of the Caenorhabditis elegans genome using ...." |
JA:K06H7.1 | |
Embryonic Phenotypes | Emb Penetrance 100 % |
Larval Phenotypes | Lvl |
Adult Phenotypes | Pvl |
Adult Phenotypes | Adl |
RNAi Reagent Name & Type | sjj_K06H7.1 (PCR reagent) |
Laboratory | JA |
Date | 2003-1-10 |
Reference | Kamath RS et al. (2003) Nature "Systemic functional analysis of the Caenorhabditis elegans genome using ...." |
PF:GL1_4E6 | |
Embryonic Phenotypes | Emb Penetrance 100 % |
Adult Phenotypes | Ste Reduced fecundity of injected worm |
Adult Phenotypes | Catastrophic one-cell arrest Multiple nuclei in 1, 2, or 4-cell embryos Osmotically/ pressure sensitive Slow cell cycle |
RNAi Reagent Name & Type | sjj_K06H7.1 (PCR reagent) |
Delivery Method | Injection |
Remark | PCR product contains flanking T7 promoter sequences |
Laboratory | KK |
Date | 2002-11-19 |
Reference | Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans" | Movies/Stacks/Images |
PF:GL1_5C1 | |
Embryonic Phenotypes | Emb Penetrance 100 % |
Adult Phenotypes | Ste Reduced fecundity of injected worm |
Adult Phenotypes | Catastrophic one-cell arrest Osmotically/ pressure sensitive Slow cell cycle |
RNAi Reagent Name & Type | sjj_C14B9.4 (PCR reagent) |
Delivery Method | Injection |
Remark | PCR product contains flanking T7 promoter sequences |
Laboratory | KK |
Date | 2002-11-19 |
Reference | Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans" | Movies/Stacks/Images |
Simmer:C14B9.4 | |
Embryonic Phenotypes | Emb Penetrance 100 % |
RNAi Reagent Name & Type | sjj_C14B9.4 (PCR reagent) |
Laboratory | NL |
Date | 2003-10-01 |
Strain | NL4256 rrf-3(pk1426) |
Reference | Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...." |
Simmer:K06H7.1 | |
Embryonic Phenotypes | Emb Penetrance 100 % |
RNAi Reagent Name & Type | sjj_K06H7.1 (PCR reagent) |
Laboratory | NL |
Date | 2003-10-01 |
Strain | NL4256 rrf-3(pk1426) |
Reference | Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...." |
TH:302e10 | |
Embryonic Phenotypes | Emb Penetrance None reported |
RNAi Reagent Name & Type | TH:302e10 (PCR reagent) |
Remark | Phenotype comment -- Confirmed with independent dsRNA against K06H7.1 (K06H7.1-RNA1); see also Chase et al. (2000). A shorter dsRNA against K06H7.1, covering exons 3 and 4 (K06H7.1-RNA2), did not result in a phenotype, suggesting inaccurate gene prediction. |
Laboratory | TH |
Date | 2000-11-16 |
Reference | Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...." |
TH:302f10 | |
Embryonic Phenotypes | Emb Penetrance None reported |
RNAi Reagent Name & Type | TH:302f10 (PCR reagent) |
Remark | Phenotype comment -- Confirmed with independent dsRNA against K06H7.1 (K06H7.1-RNA1); see also Chase et al. (2000). A shorter dsRNA against K06H7.1, covering exons 3 and 4 (K06H7.1-RNA2), did not result in a phenotype, suggesting inaccurate gene prediction. |
Laboratory | TH |
Date | 2000-11-16 |
Reference | Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...." |
Gene Plot of C14B9.4 | |