Ace View of F10B5.6 | |
F10B5.6 Details | |
Description | emb-27 encodes a tetratricopeptide repeat (TPR)-containing protein that is the C. elegans ortholog of the anaphase-promoting complex (APC) subunit APC-6 (CDC16); required maternally and paternally, EMB-27 activity is essential for proper execution of the metaphase-to-anaphase transition during meiosis (both oogenesis and spermatogenesis) and mitosis (during germline proliferation); EMB-27 activity is also critical for embryonic anterior-posterior (A-P) axis formation, production of the secreted eggshell, and normal vulval development; in establishing the embryonic A-P axis, EMB-27 likely acts upstream of the separin protease to ensure proper localization of PAR-3 and PAR-2 to the anterior and posterior cortices, respectively. |
Homology Group (KOG) | Anaphase-promoting complex (APC), Cdc16 subunit |
GO Terms | binding (GO:0005488) |
Location | II:8159386..8162495 |
RNAi Phenotypes | |
External Links | |
Associated RNAi Experiments | |
Cenix:208-a6 | |
Embryonic Phenotypes | Emb Penetrance None reported |
Adult Phenotypes | Passage Through Meiosis defective |
RNAi Reagent Name & Type | Cenix:208-a6 (PCR reagent) |
Delivery Method | Injection |
Remark | early embryonic: Passage Through Meiosis |
Laboratory | TH |
Date | 2005-3-24 |
Reference | Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...." |
JA:F10B5.6 | |
Embryonic Phenotypes | Emb Penetrance 100 % |
Adult Phenotypes | Pvl |
RNAi Reagent Name & Type | sjj_F10B5.6 (PCR reagent) |
Laboratory | JA |
Date | 2003-1-10 |
Reference | Kamath RS et al. (2003) Nature "Systemic functional analysis of the Caenorhabditis elegans genome using ...." |
PF:GL1_4G9 | |
Embryonic Phenotypes | Emb Penetrance 80 - 100 % |
Adult Phenotypes | Stp
Observed in >=10% of progeny. No developing embryos seen 48 hours after dsRNA injection Reduced fecundity of injected worm Ste |
Adult Phenotypes | Catastrophic one-cell arrest Osmotically/ pressure sensitive |
RNAi Reagent Name & Type | sjj_F10B5.6 (PCR reagent) |
Delivery Method | Injection |
Remark | PCR product contains flanking T7 promoter sequences |
Laboratory | KK |
Date | 2002-11-19 |
Reference | Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans" | Movies/Stacks/Images |
Simmer:F10B5.6 | |
Embryonic Phenotypes | Emb Penetrance 100 % |
RNAi Reagent Name & Type | sjj_F10B5.6 (PCR reagent) |
Laboratory | NL |
Date | 2003-10-01 |
Strain | NL4256 rrf-3(pk1426) |
Reference | Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...." |
Gene Plot of F10B5.6 | |