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Gene/ORF F10B5.6 (emb-27) Ace View of F10B5.6
F10B5.6 Details Help
Description  emb-27 encodes a tetratricopeptide repeat (TPR)-containing protein that is the C. elegans ortholog of the anaphase-promoting complex (APC) subunit APC-6 (CDC16); required maternally and paternally, EMB-27 activity is essential for proper execution of the metaphase-to-anaphase transition during meiosis (both oogenesis and spermatogenesis) and mitosis (during germline proliferation); EMB-27 activity is also critical for embryonic anterior-posterior (A-P) axis formation, production of the secreted eggshell, and normal vulval development; in establishing the embryonic A-P axis, EMB-27 likely acts upstream of the separin protease to ensure proper localization of PAR-3 and PAR-2 to the anterior and posterior cortices, respectively.
Homology Group (KOG)  Anaphase-promoting complex (APC), Cdc16 subunit
GO Terms  binding (GO:0005488)
Location   II:8159386..8162495
RNAi Phenotypes
Early_embryo Catastrophic one-cell arrest ; Osmotically/ pressure sensitive ; Passage Through Meiosis defective
Adult Reduced fecundity of injected worm ; Pvl ; Ste ; No developing embryos seen 48 hours after dsRNA injection ; Stp ; Stp
External Links  wormbase.org NCBI.org NBrowse
Associated RNAi Experiments View Summary
Cenix:208-a6
Embryonic Phenotypes  Emb Penetrance None reported
Adult Phenotypes  Passage Through Meiosis defective
RNAi Reagent Name & Type Cenix:208-a6 (PCR reagent)
Delivery Method  Injection
Remark  early embryonic: Passage Through Meiosis
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
JA:F10B5.6
Embryonic Phenotypes  Emb Penetrance 100 %
Adult Phenotypes  Pvl
RNAi Reagent Name & Type sjj_F10B5.6 (PCR reagent)
Laboratory  JA
Date  2003-1-10
Reference  Kamath RS et al. (2003) Nature "Systemic functional analysis of the Caenorhabditis elegans genome using ...."
PF:GL1_4G9
Embryonic Phenotypes  Emb Penetrance 80 - 100 %
Adult Phenotypes  Stp Observed in >=10% of progeny.
No developing embryos seen 48 hours after dsRNA injection
Reduced fecundity of injected worm
Ste
Adult Phenotypes  Catastrophic one-cell arrest
Osmotically/ pressure sensitive
RNAi Reagent Name & Type sjj_F10B5.6 (PCR reagent)
Delivery Method  Injection
Remark  PCR product contains flanking T7 promoter sequences
Laboratory  KK
Date  2002-11-19
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
Movies/Stacks/Images  /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif
Simmer:F10B5.6
Embryonic Phenotypes  Emb Penetrance 100 %
RNAi Reagent Name & Type sjj_F10B5.6 (PCR reagent)
Laboratory  NL
Date  2003-10-01
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
Gene Plot of F10B5.6 Browse using GBrowse
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