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Gene/ORF F18A1.5 Ace View of F18A1.5
F18A1.5 Details Help
Description  F18A1.5 encodes the C. elegans ortholog of the replication protein A (RPA) large subunit; by homology, the F18A1.5 gene product is predicted to function as a DNA binding and oligonucleotide oligosaccharide binding (OB) protein that is required for DNA replication, repair, and recombination; large-scale RNAi screens indicate that the product of F18A1.5 is essential for embryonic development, and that in yeast two-hybrid assays interacts with the product of M04F3.1, the RPA medium subunit; in situ hybridization studies indicate that F18A1.5 transcripts are expressed in germ cells.
Homology Group (KOG)  Single-stranded DNA-binding replication protein A (RPA), large (70 kD) subunit and related ssDNA-binding proteins
GO Terms  nucleic acid binding (GO:0003676) ; DNA binding (GO:0003677) ; DNA replication (GO:0006260) ; nucleus (GO:0005634)
Location   II:7664096..7667426
RNAi Phenotypes
Early_embryo Exaggerated asynchrony ; Sister Chromatid Separation abnormal (Cross-eyed) ; Multiple maternal pronuclei ; Aberrant nuclear reassembly ; Ectopic cleavage furrows ; Slow cell cycle ; Excessive blebbing ; Abnormal size of pronucleus ; Aberrant cell-cell contacts at the four-cell stage
External Links  wormbase.org NCBI.org NBrowse
Associated RNAi Experiments View Summary
Cenix:204-f5
Embryonic Phenotypes  Emb Penetrance None reported
Adult Phenotypes  Sister Chromatid Separation abnormal (Cross-eyed)
RNAi Reagent Name & Type Cenix:204-f5 (PCR reagent)
Delivery Method  Injection
Remark  early embryonic: Sister Chromatid Separation (Cross-eyed)
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
JA:F18A1.5
Embryonic Phenotypes  Emb Penetrance 100 %
RNAi Reagent Name & Type sjj_F18A1.5 (PCR reagent)
Laboratory  JA
Date  2003-1-10
Reference  Kamath RS et al. (2003) Nature "Systemic functional analysis of the Caenorhabditis elegans genome using ...."
PF:GL1_5G7
Embryonic Phenotypes  Emb Penetrance 100 %
Adult Phenotypes  Aberrant cell-cell contacts at the four-cell stage
Aberrant nuclear reassembly
Abnormal size of pronucleus
Ectopic cleavage furrows
Exaggerated asynchrony
Excessive blebbing
Multiple maternal pronuclei
Slow cell cycle
RNAi Reagent Name & Type sjj_F18A1.5 (PCR reagent)
Delivery Method  Injection
Remark  PCR product contains flanking T7 promoter sequences
Laboratory  KK
Date  2002-11-19
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
Movies/Stacks/Images  /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif
Simmer:F18A1.5
Embryonic Phenotypes  Emb Penetrance 100 %
RNAi Reagent Name & Type sjj_F18A1.5 (PCR reagent)
Laboratory  NL
Date  2003-10-01
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
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