Ace View of F18A1.5 | |
F18A1.5 Details | |
Description | F18A1.5 encodes the C. elegans ortholog of the replication protein A (RPA) large subunit; by homology, the F18A1.5 gene product is predicted to function as a DNA binding and oligonucleotide oligosaccharide binding (OB) protein that is required for DNA replication, repair, and recombination; large-scale RNAi screens indicate that the product of F18A1.5 is essential for embryonic development, and that in yeast two-hybrid assays interacts with the product of M04F3.1, the RPA medium subunit; in situ hybridization studies indicate that F18A1.5 transcripts are expressed in germ cells. |
Homology Group (KOG) | Single-stranded DNA-binding replication protein A (RPA), large (70 kD) subunit and related ssDNA-binding proteins |
GO Terms | nucleic acid binding (GO:0003676) ; DNA binding (GO:0003677) ; DNA replication (GO:0006260) ; nucleus (GO:0005634) |
Location | II:7664096..7667426 |
RNAi Phenotypes | |
External Links | |
Associated RNAi Experiments | |
Cenix:204-f5 | |
Embryonic Phenotypes | Emb Penetrance None reported |
Adult Phenotypes | Sister Chromatid Separation abnormal (Cross-eyed) |
RNAi Reagent Name & Type | Cenix:204-f5 (PCR reagent) |
Delivery Method | Injection |
Remark | early embryonic: Sister Chromatid Separation (Cross-eyed) |
Laboratory | TH |
Date | 2005-3-24 |
Reference | Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...." |
JA:F18A1.5 | |
Embryonic Phenotypes | Emb Penetrance 100 % |
RNAi Reagent Name & Type | sjj_F18A1.5 (PCR reagent) |
Laboratory | JA |
Date | 2003-1-10 |
Reference | Kamath RS et al. (2003) Nature "Systemic functional analysis of the Caenorhabditis elegans genome using ...." |
PF:GL1_5G7 | |
Embryonic Phenotypes | Emb Penetrance 100 % |
Adult Phenotypes | Aberrant cell-cell contacts at the four-cell stage Aberrant nuclear reassembly Abnormal size of pronucleus Ectopic cleavage furrows Exaggerated asynchrony Excessive blebbing Multiple maternal pronuclei Slow cell cycle |
RNAi Reagent Name & Type | sjj_F18A1.5 (PCR reagent) |
Delivery Method | Injection |
Remark | PCR product contains flanking T7 promoter sequences |
Laboratory | KK |
Date | 2002-11-19 |
Reference | Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans" | Movies/Stacks/Images |
Simmer:F18A1.5 | |
Embryonic Phenotypes | Emb Penetrance 100 % |
RNAi Reagent Name & Type | sjj_F18A1.5 (PCR reagent) |
Laboratory | NL |
Date | 2003-10-01 |
Strain | NL4256 rrf-3(pk1426) |
Reference | Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...." |
Gene Plot of F18A1.5 | |