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Gene/ORF F31E3.3 (rfc-4) Ace View of F31E3.3
F31E3.3 Details Help
Homology Group (KOG)  Replication factor C, subunit RFC4
GO Terms  nucleotide binding (GO:0000166) ; nucleoside triphosphatase activity (GO:0017111) ; ATP binding (GO:0005524) ; DNA binding (GO:0003677) ; DNA replication (GO:0006260) ; protein complex (GO:0043234)
Location   III:6971791..6973986
RNAi Phenotypes
Early_embryo Exaggerated asynchrony ; Pace of P-Lineage abnormal ; EMS fails to extend anteriorly ; Multiple nuclei in 1, 2, or 4-cell embryos ; Ectopic cleavage furrows ; Cytokinesis defect ; Excessive blebbing ; Aberrant cell-cell contacts at the four-cell stage ; Nuclei reform next to cell division remnant
Larva Gro
Adult Stp
External Links  wormbase.org NCBI.org NBrowse
Associated RNAi Experiments View Summary
Cenix:306-g12
Embryonic Phenotypes  Emb Penetrance None reported
Adult Phenotypes  Pace of P-Lineage abnormal
RNAi Reagent Name & Type Cenix:306-g12 (PCR reagent)
Delivery Method  Injection
Remark  early embryonic: Pace of P-Lineage
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
Cenix:340-h10
Embryonic Phenotypes  Emb Penetrance None reported
RNAi Reagent Name & Type Cenix:340-h10 (PCR reagent)
Delivery Method  Injection
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
JA:F31E3.3
Embryonic Phenotypes  Emb Penetrance 40 %
Larval Phenotypes  Gro
RNAi Reagent Name & Type sjj_F31E3.3 (PCR reagent)
Laboratory  JA
Date  2003-1-10
Reference  Kamath RS et al. (2003) Nature "Systemic functional analysis of the Caenorhabditis elegans genome using ...."
PF:GL1_4B5
Embryonic Phenotypes  Emb Penetrance 80 - 100 %
Adult Phenotypes  Stp Observed in >=10% of progeny.
Adult Phenotypes  Aberrant cell-cell contacts at the four-cell stage
Cytokinesis defect
Ectopic cleavage furrows
EMS fails to extend anteriorly
Exaggerated asynchrony
Excessive blebbing
Multiple nuclei in 1, 2, or 4-cell embryos
Nuclei reform next to cell division remnant
RNAi Reagent Name & Type sjj_F31E3.3 (PCR reagent)
Delivery Method  Injection
Remark  PCR product contains flanking T7 promoter sequences
Laboratory  KK
Date  2002-11-19
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
Movies/Stacks/Images  /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif
TH:306g12
Embryonic Phenotypes  Emb Penetrance None reported
RNAi Reagent Name & Type TH:306g12 (PCR reagent)
Remark  Phenotype comment -- Confirmed with independent dsRNA (F31E3.3-RNA2; similar phenotype).
Laboratory  TH
Date  2000-11-16
Reference  Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...."
TH:340h10
Embryonic Phenotypes  Emb Penetrance None reported
RNAi Reagent Name & Type TH:340h10 (PCR reagent)
Remark  Phenotype comment -- Confirmed with independent dsRNA (F31E3.3-RNA2; similar phenotype).
Laboratory  TH
Date  2000-11-16
Reference  Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...."
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