Go to RNAiDB Home Adult Wild-type C. elegans Movie Wild-type C. elegans Movie Wild-type C. elegans Movie Wild-type C. elegans Movie
 
Link to F35G12.10RNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentgeneMap
Gene/ORF F35G12.10 (asb-1) Ace View of F35G12.10
F35G12.10 Details Help
Description  asb-1 encodes a homolog of ATP synthase B, closely similar to its paralog ASB-2, that is required for embryonic viability and for the normal proliferation of germline cells; asb-1 is preferentially expressed in germline cells, while RNAi of asb-1 causes embryonic lethality and sterility with a defective progression to meiosis.
Homology Group (KOG)  Mitochondrial F1F0-ATP synthase, subunit b/ATP4
GO Terms  hydrogen-transporting ATP synthase activity, rotational mechanism (GO:0046933) ; hydrogen-transporting ATPase activity, rotational mechanism (GO:0046961) ; ATP synthesis coupled proton transport (GO:0015986) ; proton-transporting two-sector ATPase complex (GO:0016469)
Location   III:4594108..4596186
RNAi Phenotypes
Larva Gro
Adult Ste ; Ste ; Stp
External Links  wormbase.org NCBI.org NBrowse
Associated RNAi Experiments View Summary
Cenix:300-e8
Embryonic Phenotypes  Emb Penetrance None reported
RNAi Reagent Name & Type Cenix:300-e8 (PCR reagent)
Delivery Method  Injection
Remark  early embryonic: All embryos show a very slow overall pace of events.
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
Cenix:341-c1
RNAi Reagent Name & Type Cenix:341-c1 (PCR reagent)
Delivery Method  Injection
Remark  early embryonic: Embryos have a very slow pace of developement. There is also a thin area devoid of yolk granules surrounding the spidle set up.
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
PF:GL1_3B1
Adult Phenotypes  WT
RNAi Reagent Name & Type sjj_F35G12.10 (PCR reagent)
Delivery Method  Injection
Remark  PCR product contains flanking T7 promoter sequences
Laboratory  KK
Date  2002-11-19
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
Simmer:F35G12.10
Embryonic Phenotypes  Emb Penetrance 50 - 80 %
Larval Phenotypes  Gro
Adult Phenotypes  Stp
RNAi Reagent Name & Type sjj_F35G12.10 (PCR reagent)
Laboratory  NL
Date  2003-10-01
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
TH:300e8
Embryonic Phenotypes  Emb Penetrance None reported
RNAi Reagent Name & Type TH:300e8 (PCR reagent)
Remark  Phenotype comment -- Confirmed with independent dsRNA (F35G12.10-RNA2; similar DIC phenotype, but progeny test was not embryonic lethal).
Laboratory  TH
Date  2000-11-16
Reference  Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...."
TH:341c1
Embryonic Phenotypes  Emb Penetrance None reported
RNAi Reagent Name & Type TH:341c1 (PCR reagent)
Remark  Phenotype comment -- Confirmed with independent dsRNA (F35G12.10-RNA2; similar DIC phenotype, but progeny test was not embryonic lethal).
Laboratory  TH
Date  2000-11-16
Reference  Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...."
Gene Plot of F35G12.10 Browse using GBrowse
Gene Map