| Ace View of F35G12.8 | |
| F35G12.8 Details | |
| Description | The smc-4 gene encodes a homolog of the SMC4 subunit of mitotic condensin; SMC-4 acts with MIX-1 to enable chromosome segregation. |
| Homology Group (KOG) | Structural maintenance of chromosome protein 4 (chromosome condensation complex Condensin, subunit C) |
| GO Terms | ATP binding (GO:0005524) ; ATPase activity (GO:0016887) ; chromosome organization and biogenesis (GO:0051276) ; chromosome (GO:0005694) ; protein binding (GO:0005515) |
| Location | III:4587071..4593474 |
| RNAi Phenotypes | |
| External Links |    |
| Associated RNAi Experiments | |
| Cenix:311-h1 | |
| Adult Phenotypes | WT |
| RNAi Reagent Name & Type | Cenix:311-h1 (PCR reagent) |
| Delivery Method | Injection |
| Remark | wild type |
| Laboratory | TH |
| Date | 2005-3-24 |
| Reference | Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...." |
| TH:310G8 | |
| Embryonic Phenotypes | Emb Penetrance None reported |
| RNAi Reagent Name & Type | TH:310G8 (PCR reagent) |
| Remark | Phenotype comment -- Phenotype confirmed by second independent RNA (RNA2). |
| Laboratory | TH |
| Date | 2000-11-16 |
| Reference | Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...." |
| Cenix:300-b1 | |
| Embryonic Phenotypes | Emb Penetrance None reported |
| Adult Phenotypes | Sister Chromatid Separation abnormal (Cross-eyed) |
| RNAi Reagent Name & Type | Cenix:300-b1 (PCR reagent) |
| Delivery Method | Injection |
| Remark | early embryonic: Sister Chromatid Separation (Cross-eyed) |
| Laboratory | TH |
| Date | 2005-3-24 |
| Reference | Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...." |
| Cenix:340-c12 | |
| Embryonic Phenotypes | Emb Penetrance None reported |
| Adult Phenotypes | Sister Chromatid Separation abnormal (Cross-eyed) |
| RNAi Reagent Name & Type | Cenix:340-c12 (PCR reagent) |
| Delivery Method | Injection |
| Remark | early embryonic: Sister Chromatid Separation (Cross-eyed) |
| Laboratory | TH |
| Date | 2005-3-24 |
| Reference | Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...." |
| JA:F35G12.8 | |
| Embryonic Phenotypes | Emb Penetrance 100 % |
| Adult Phenotypes | Pvl |
| RNAi Reagent Name & Type | sjj_F35G12.8 (PCR reagent) |
| Laboratory | JA |
| Date | 2003-1-10 |
| Reference | Kamath RS et al. (2003) Nature "Systemic functional analysis of the Caenorhabditis elegans genome using ...." |
| PF:GL1_4C11 | |
| Embryonic Phenotypes | Emb Penetrance 80 - 100 % |
| Larval Phenotypes | Lva
Observed in >=10% of progeny. |
| Adult Phenotypes | ABa, ABp and EMS divide synchronously Aberrant AB or P1 spindle orientation Cytokinesis defect Delayed P0 spindle rotation Division axis of ABa or ABp aberrant Ectopic cleavage furrows Multiple cavities Multiple nuclei in 1, 2, or 4-cell embryos Nuclei reform next to cell division remnant P2 and EMS divide synchronously Pronuclei meet more centrally |
| RNAi Reagent Name & Type | sjj_F35G12.8 (PCR reagent) |
| Delivery Method | Injection |
| Remark | PCR product contains flanking T7 promoter sequences |
| Laboratory | KK |
| Date | 2002-11-19 |
| Reference | Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans" | Movies/Stacks/Images |  |
| Simmer:F35G12.8 | |
| Embryonic Phenotypes | Emb Penetrance 100 % |
| RNAi Reagent Name & Type | sjj_F35G12.8 (PCR reagent) |
| Laboratory | NL |
| Date | 2003-10-01 |
| Strain | NL4256 rrf-3(pk1426) |
| Reference | Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...." |
| TH:300b1 | |
| Embryonic Phenotypes | Emb Penetrance None reported |
| RNAi Reagent Name & Type | TH:300b1 (PCR reagent) |
| Remark | Phenotype comment -- Confirmed with independent dsRNA (F35G12.8-RNA2; similar phenotype). |
| Laboratory | TH |
| Date | 2000-11-16 |
| Reference | Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...." |
| TH:340c12 | |
| Embryonic Phenotypes | Emb Penetrance None reported |
| RNAi Reagent Name & Type | TH:340c12 (PCR reagent) |
| Remark | Phenotype comment -- Confirmed with independent dsRNA (F35G12.8-RNA2; similar phenotype). |
| Laboratory | TH |
| Date | 2000-11-16 |
| Reference | Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...." |
| Cenix:329-g10 | |
| Adult Phenotypes | Larval Lethal-Early (L1/L2) |
| RNAi Reagent Name & Type | Cenix:329-g10 (PCR reagent) |
| Delivery Method | Injection |
| Remark | post-embryonic: Larval Lethal-Early (L1/L2) |
| Laboratory | TH |
| Date | 2005-3-24 |
| Reference | Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...." |
| Cenix:310-g8 | |
| Embryonic Phenotypes | Emb Penetrance None reported |
| Adult Phenotypes | WT_postembryonic_morphology |
| RNAi Reagent Name & Type | Cenix:310-g8 (PCR reagent) |
| Delivery Method | Injection |
| Laboratory | TH |
| Date | 2005-3-24 |
| Reference | Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...." |
| Gene Plot of F35G12.8 | |
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