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Gene/ORF F49E11.1 (mbk-2) Ace View of F49E11.1
F49E11.1 Details Help
Description  mbk-2 encodes one of two C. elegans members of the DYRK (dual-specificity Yak1-related kinase) family of proteins that includes S. cerevisiae Yak1 and the Drosophila minibrain and DYRK2 kinases; MBK-2 activity is required maternally for the egg-to-embryo transition that occurs during the earliest stages of embryonic development; specifically, MBK-2 is required for: 1) posterior localization of the germ plasm components PIE-1, POS-1, and PGL-1, and 2) postfertilization degradation of a subset of maternal proteins including the MEI-1 and MEI-2 meiosis-specific katanin subunits, the OMA-1 oocyte maturation factor, and residual PIE-1 that remains anteriorly localized after its normal posterior segregation; genetic analyses suggest that, in regulating the segregation and degradation of maternal proteins, mbk-2 lies downstream of the initial embryonic polarity cues established by the PAR/MEX proteins; MBK-2, whose activity appears to depend upon progression through the meiotic divisions, is expressed uniformly in the cortex of oocytes and newly fertilized zygotes; in later zygotes, just prior to the second meiotic division, MBK-2 becomes localized to discrete cortical foci, and by the first mitosis it is found predominantly on centrosomes and chromosomes; MBK-2 is also associated with P granules in the germline blastomeres P2, P3, and P4.
Homology Group (KOG)  Dual-specificity tyrosine-phosphorylation regulated kinase
GO Terms  autophosphorylation (GO:0046777) ; peptidyl-serine phosphorylation (GO:0018105) ; peptidyl-threonine phosphorylation (GO:0018107) ; peptidyl-tyrosine phosphorylation (GO:0018108) ; positive regulation of protein catabolism (GO:0045732) ; embryonic development (sensu Animalia) (GO:0009792) ; cytokinesis after mitosis (GO:0000281) ; embryonic pattern specification (GO:0009880) ; asymmetric protein localization involved in cell fate commitment (GO:0045167) ; microtubule-based process (GO:0007017) ; cell cortex (GO:0005938) ; cytoplasm (GO:0005737) ; centrosome (GO:0005813) ; chromosome (GO:0005694) ; P granule (GO:0043186) ; protein serine/threonine kinase activity (GO:0004674) ; protein-tyrosine kinase activity (GO:0004713) ; ATP binding (GO:0005524) ; protein kinase activity (GO:0004672) ; protein amino acid phosphorylation (GO:0006468)
Location   IV:13008705..13036316
RNAi Phenotypes
Early_embryo P0 spindle does not rotate ; Aberrant AB or P1 spindle orientation ; Exaggerated pseudocleavage ; EMS fails to extend anteriorly ; Multiple nuclei in 1, 2, or 4-cell embryos ; Ectopic cleavage furrows ; Cytokinesis defect ; Excessive blebbing ; Pronuclear breakdown asynchronous ; Pnm ; Aberrant cell-cell contacts at the four-cell stage ; Loose mitotic furrow
Adult Dpy ; Rup ; Stp
External Links  wormbase.org NCBI.org NBrowse
Associated RNAi Experiments View Details
Wild Type RNAi Experiments
JA:F49E11.1 WT
Experiments with Non-Wild Phenotypes
PF:GL1_3A1 Aberrant AB or P1 spindle orientation ; Aberrant cell-cell contacts at the four-cell stage ; Cytokinesis defect ; Dpy ; Ectopic cleavage furrows ; Emb ; EMS fails to extend anteriorly ; Exaggerated pseudocleavage ; Excessive blebbing ; Loose mitotic furrow ; Multiple nuclei in 1, 2, or 4-cell embryos ; P0 spindle does not rotate ; Pronuclear breakdown asynchronous ; Rup ; Stp
Cenix:10-e8 Emb ; Pnm
Gene Plot of F49E11.1 Browse using GBrowse
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