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Gene/ORF F56H1.4 (rpt-5) Ace View of F56H1.4
F56H1.4 Details Help
Description  rpt-5 encodes a triple A ATPase subunit of the 26S proteasome's 19S regulatory particle (RP) base subcomplex; RPT-5 is required for embryonic, larval, and germline development and by homology, is predicted to function in unfolding protein substrates and translocating them into the core proteolytic particle (CP) of the proteasome.
Homology Group (KOG)  26S proteasome regulatory complex, ATPase RPT5
GO Terms  hydrolase activity (GO:0016787) ; protein catabolism (GO:0030163) ; nucleus (GO:0005634) ; cytoplasm (GO:0005737) ; nucleotide binding (GO:0000166) ; nucleoside triphosphatase activity (GO:0017111) ; ATP binding (GO:0005524)
Location   I:5741471..5743976
RNAi Phenotypes
Early_embryo Catastrophic one-cell arrest ; Ocs ; Osmotically/ pressure sensitive ; Passage Through Meiosis defective
Larva Lvl
Adult Reduced fecundity of injected worm ; Ste ; Ste ; Ste ; No developing embryos seen 48 hours after dsRNA injection
External Links  wormbase.org NCBI.org NBrowse
Associated RNAi Experiments View Summary
Cenix:148-h6
Embryonic Phenotypes  Emb Penetrance None reported
Adult Phenotypes  Passage Through Meiosis defective
RNAi Reagent Name & Type Cenix:148-h6 (PCR reagent)
Delivery Method  Injection
Remark  early embryonic: Passage Through Meiosis
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
JA:F56H1.4
Embryonic Phenotypes  Emb Penetrance 100 %
Adult Phenotypes  Ste brood size range (1 out of 5)
Adult Phenotypes  Ocs 1-12 cell arrest
RNAi Reagent Name & Type sjj_F56H1.4 (PCR reagent)
Laboratory  JA
Date  2000-11-16
Reference  Fraser AG et al. (2000) Nature "Functional genomic analysis of C. elegans chromosome I by systematic RNA ...."
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PF:GL1_4E9
Embryonic Phenotypes  Emb Penetrance 100 %
Adult Phenotypes  Ste
No developing embryos seen 48 hours after dsRNA injection
Reduced fecundity of injected worm
Adult Phenotypes  Catastrophic one-cell arrest
Osmotically/ pressure sensitive
RNAi Reagent Name & Type sjj_F56H1.4 (PCR reagent)
Delivery Method  Injection
Remark  PCR product contains flanking T7 promoter sequences
Laboratory  KK
Date  2002-11-19
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
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Simmer:F56H1.4:Screen_A
Embryonic Phenotypes  Emb Penetrance 100 %
Adult Phenotypes  Ste brood size (6 out of 10)
RNAi Reagent Name & Type sjj_F56H1.4 (PCR reagent)
Laboratory  NL
Date  2003-10-01
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
Simmer:F56H1.4:Screen_B
Embryonic Phenotypes  Emb Penetrance 100 %
RNAi Reagent Name & Type sjj_F56H1.4 (PCR reagent)
Laboratory  NL
Date  2003-10-01
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
Gene Plot of F56H1.4 Browse using GBrowse
Gene Map