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Gene/ORF F58A4.4 (pri-1) Ace View of F58A4.4
F58A4.4 Details Help
Description  pri-1 encodes a homolog of the DNA polymerase alpha-primase subunit D that is required in embryos for the normal timing of embryonic cell divisions, much as DIV-1 is.
Homology Group (KOG)  Eukaryotic-type DNA primase, catalytic (small) subunit
Location   III:9611810..9614577
RNAi Phenotypes
Early_embryo Exaggerated asynchrony ; Exaggerated asynchrony ; Sister Chromatid Separation abnormal (Cross-eyed) ; Sister Chromatid Separation abnormal (Cross-eyed) ; Pace of P-Lineage abnormal ; Multiple cavities ; Multiple nuclei in 1, 2, or 4-cell embryos ; Division axis of ABa or ABp aberrant ; Ectopic cleavage furrows ; Ectopic cleavage furrows ; Slow cell cycle ; Excessive blebbing ; Excessive blebbing ; Large cytoplasmic granules ; Aberrant cell-cell contacts at the four-cell stage ; Nuclei reform next to cell division remnant ; Nuclei reform next to cell division remnant
Larva Lva
External Links  wormbase.org NCBI.org NBrowse
Associated RNAi Experiments View Summary
Cenix:335-f2
Embryonic Phenotypes  Emb Penetrance None reported
Adult Phenotypes  Sister Chromatid Separation abnormal (Cross-eyed)
RNAi Reagent Name & Type Cenix:335-f2 (PCR reagent)
Delivery Method  Injection
Remark  early embryonic: Sister Chromatid Separation (Cross-eyed)
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
TH:335f2
Embryonic Phenotypes  Emb Penetrance None reported
RNAi Reagent Name & Type TH:335f2 (PCR reagent)
Remark  Phenotype comment -- Confirmed with independent dsRNA (F58A4.4 -RNA2; similar phenotype).
Laboratory  TH
Date  2000-11-16
Reference  Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...."
Cenix:340-c4
Embryonic Phenotypes  Emb Penetrance None reported
Adult Phenotypes  Sister Chromatid Separation abnormal (Cross-eyed)
RNAi Reagent Name & Type Cenix:340-c4 (PCR reagent)
Delivery Method  Injection
Remark  early embryonic: Sister Chromatid Separation (Cross-eyed)
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
PF:GL1_7D9
Embryonic Phenotypes  Emb Penetrance 100 %
Adult Phenotypes  Division axis of ABa or ABp aberrant
Ectopic cleavage furrows
Exaggerated asynchrony
Excessive blebbing
Large cytoplasmic granules
Multiple cavities
Multiple nuclei in 1, 2, or 4-cell embryos
Nuclei reform next to cell division remnant
Slow cell cycle
RNAi Reagent Name & Type sjj_F58A4.4 (PCR reagent)
Delivery Method  Injection
Remark  PCR product contains flanking T7 promoter sequences
Laboratory  KK
Date  2002-11-19
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
Movies/Stacks/Images  /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif
Simmer:F58A4.4
Embryonic Phenotypes  Emb Penetrance 100 %
RNAi Reagent Name & Type sjj_F58A4.4 (PCR reagent)
Laboratory  NL
Date  2003-10-01
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
TH:340c4
Embryonic Phenotypes  Emb Penetrance None reported
RNAi Reagent Name & Type TH:340c4 (PCR reagent)
Remark  Phenotype comment -- Confirmed with independent dsRNA (F58A4.4 -RNA2; similar phenotype).
Laboratory  TH
Date  2000-11-16
Reference  Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...."
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