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Gene/ORF K08E7.3 (let-99) Ace View of K08E7.3
K08E7.3 Details Help
Description  let-99 encodes a protein with a DEP domain (Domain found in Dishevelled, Egl-10, and Pleckstrin); LET-99 has no obvious homologs in non-nematodes, but has a truncated paralog (LRG-1) in C. elegans; LET-99 is required for the mitotic spindle to be properly oriented with respect to the axis of cellular polarity, both during anterior migration and rotation of the nuclear-centrosome complex and during anaphase; let-99 mutants exhibit hyperactive nuclear movement and abnormal anaphase spindle pole behavior; LET-99 is mislocalized in par-2 and par-3 mutants; like other proteins containing DEP domains, LET-99 may act as part of a G protein signalling pathway (e.g., the one controlling spindle position).
GO Terms  intracellular signaling cascade (GO:0007242)
Location   IV:12567427..12570976
RNAi Phenotypes
Early_embryo P0 spindle does not rotate ; P0 spindle does not rotate ; Pronuclei meet more centrally ; P2 and EMS divide synchronously ; Aberrant AB or P1 spindle orientation ; P1 aster abnormal ; Division axis of ABa or ABp aberrant ; Division axis of ABa or ABp aberrant ; Synchronous second division ; Delayed P0 spindle rotation ; Delayed P0 spindle rotation ; Large cytoplasmic granules ; Pnm ; Pnm ; Pnm ; Aberrant cell-cell contacts at the four-cell stage ; Aberrant cell-cell contacts at the four-cell stage ; Nuclei reform next to cell division remnant ; P0 spindle positioning defect ; P0 spindle positioning defect
Larva Lva
Adult Stp ; Stp
External Links  wormbase.org NCBI.org NBrowse
Associated RNAi Experiments View Details
Experiments with Non-Wild Phenotypes
PF:GL1_6E4 Aberrant AB or P1 spindle orientation ; Aberrant cell-cell contacts at the four-cell stage ; Delayed P0 spindle rotation ; Division axis of ABa or ABp aberrant ; Emb ; Nuclei reform next to cell division remnant ; P0 spindle does not rotate ; P0 spindle positioning defect ; P1 aster abnormal ; P2 and EMS divide synchronously ; Stp
Cenix:186-d3 Emb ; Pnm
TH:198-b9
Gene Plot of K08E7.3 Browse using GBrowse
Gene Map