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Gene/ORF K08E7.3 (let-99) Ace View of K08E7.3
K08E7.3 Details Help
Description  let-99 encodes a protein with a DEP domain (Domain found in Dishevelled, Egl-10, and Pleckstrin); LET-99 has no obvious homologs in non-nematodes, but has a truncated paralog (LRG-1) in C. elegans; LET-99 is required for the mitotic spindle to be properly oriented with respect to the axis of cellular polarity, both during anterior migration and rotation of the nuclear-centrosome complex and during anaphase; let-99 mutants exhibit hyperactive nuclear movement and abnormal anaphase spindle pole behavior; LET-99 is mislocalized in par-2 and par-3 mutants; like other proteins containing DEP domains, LET-99 may act as part of a G protein signalling pathway (e.g., the one controlling spindle position).
GO Terms  intracellular signaling cascade (GO:0007242)
Location   IV:12567427..12570976
RNAi Phenotypes
Early_embryo P0 spindle does not rotate ; P0 spindle does not rotate ; Pronuclei meet more centrally ; P2 and EMS divide synchronously ; Aberrant AB or P1 spindle orientation ; P1 aster abnormal ; Division axis of ABa or ABp aberrant ; Division axis of ABa or ABp aberrant ; Synchronous second division ; Delayed P0 spindle rotation ; Delayed P0 spindle rotation ; Large cytoplasmic granules ; Pnm ; Pnm ; Pnm ; Aberrant cell-cell contacts at the four-cell stage ; Aberrant cell-cell contacts at the four-cell stage ; Nuclei reform next to cell division remnant ; P0 spindle positioning defect ; P0 spindle positioning defect
Larva Lva
Adult Stp ; Stp
External Links  wormbase.org NCBI.org NBrowse
Associated RNAi Experiments View Summary
Cenix:186-d3
Embryonic Phenotypes  Emb Penetrance None reported
Adult Phenotypes  Pnm
RNAi Reagent Name & Type Cenix:186-d3 (PCR reagent)
Delivery Method  Injection
Remark  early embryonic: Pronuclear Migration
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
PF:GL1_6E4
Embryonic Phenotypes  Emb Penetrance 80 - 100 %
Adult Phenotypes  Stp Observed in >=10% of progeny.
Adult Phenotypes  Aberrant AB or P1 spindle orientation
Aberrant cell-cell contacts at the four-cell stage
Delayed P0 spindle rotation
Division axis of ABa or ABp aberrant
Nuclei reform next to cell division remnant
P0 spindle does not rotate
P0 spindle positioning defect
P1 aster abnormal
P2 and EMS divide synchronously
RNAi Reagent Name & Type sjj_K08E7.3 (PCR reagent)
Delivery Method  Injection
Remark  PCR product contains flanking T7 promoter sequences
Laboratory  KK
Date  2002-11-19
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
Movies/Stacks/Images  /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif
TH:198-b9
RNAi Reagent Name & Type TH:198-b9 (PCR reagent)
Gene Plot of K08E7.3 Browse using GBrowse
Gene Map