| Ace View of M7.1 | |||||||||
| M7.1 Details | |||||||||
| Description | let-70 encodes a class I E2 ubiquitin conjugating enzyme; let-70 was identified in screens for essential genes and phenotypic analyses indicate that loss of let-70 activity results in embryonic and larval lethality with defects in sarcomere assembly; purified LET-70 can stimulate the self-ubiquitylation activities of CHN-1 and UFD-2, E4 ubiquitin conjugation factors and, in addition, can stimulate the CHN-1- and UFD-2-dependent multiubiquitylation of UNC-45, a myosin-directed chaperone; in this manner, LET-70 may regulate UNC-45 degradation and myosin assembly; RNA blot analyses and a let-70-lacZ reporter fusion reveal that let-70 is abundantly expressed at all stages of development, including the dauer larval stage; strong staining is observed in most somatic tissues until adult stages, when expression generally becomes restricted to the nervous system. | ||||||||
| Homology Group (KOG) | Ubiquitin-protein ligase |
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| GO Terms | ubiquitin-like activating enzyme activity (GO:0008642) ; protein modification (GO:0006464) ; ubiquitin cycle (GO:0006512) | ||||||||
| Location | IV:11080655..11082625 | ||||||||
| RNAi Phenotypes |
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| External Links |    |
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| Associated RNAi Experiments | |||||||||
| Cenix:186-e7 | |||||||||
| Embryonic Phenotypes | Emb Penetrance None reported | ||||||||
| Adult Phenotypes | Complex Phenotype |
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| RNAi Reagent Name & Type | Cenix:186-e7 (PCR reagent) | ||||||||
| Delivery Method | Injection | ||||||||
| Remark | early embryonic: Complex Phenotype | ||||||||
| Laboratory | TH | ||||||||
| Date | 2005-3-24 | ||||||||
| Reference | Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...." | ||||||||
| JA:M7.1 | |||||||||
| Embryonic Phenotypes | Emb Penetrance 100 % | ||||||||
| Adult Phenotypes | Pvl |
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| Adult Phenotypes | Adl |
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| RNAi Reagent Name & Type | sjj_M7.1 (PCR reagent) | ||||||||
| Laboratory | JA | ||||||||
| Date | 2003-1-10 | ||||||||
| Reference | Kamath RS et al. (2003) Nature "Systemic functional analysis of the Caenorhabditis elegans genome using ...." | ||||||||
| Simmer:M7.1 | |||||||||
| Embryonic Phenotypes | Emb Penetrance 100 % | ||||||||
| RNAi Reagent Name & Type | sjj_M7.1 (PCR reagent) | ||||||||
| Laboratory | NL | ||||||||
| Date | 2003-10-01 | ||||||||
| Strain | NL4256 rrf-3(pk1426) | ||||||||
| Reference | Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...." | ||||||||
| WBRNAi00060823 | |||||||||
| Adult Phenotypes | aldicarb_resistant
Animals displayed resistance to paralysis on aldicarb in 6 out of 8 trials. |
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| RNAi Reagent Name & Type | sjj_M7.1 (PCR reagent) | ||||||||
| Delivery Method | Bacterial_feeding | ||||||||
| Laboratory | KP | ||||||||
| Date | 2005-5-09 | ||||||||
| Reference | Sieburth D et al. (2005) Nature "Systematic analysis of genes required for synapse structure and ...." | ||||||||
| WBRNAi00060824 | |||||||||
| Adult Phenotypes | WT |
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| RNAi Reagent Name & Type | sjj_M7.1 (PCR reagent) | ||||||||
| Delivery Method | Bacterial_feeding | ||||||||
| Laboratory | KP | ||||||||
| Date | 2005-5-09 | ||||||||
| Reference | Sieburth D et al. (2005) Nature "Systematic analysis of genes required for synapse structure and ...." | ||||||||
| WBRNAi00060825 | |||||||||
| Larval Phenotypes | Let |
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| RNAi Reagent Name & Type | sjj_M7.1 (PCR reagent) | ||||||||
| Delivery Method | Bacterial_feeding | ||||||||
| Laboratory | KP | ||||||||
| Date | 2005-5-09 | ||||||||
| Reference | Sieburth D et al. (2005) Nature "Systematic analysis of genes required for synapse structure and ...." | ||||||||
| WBRNAi00060826 | |||||||||
| Larval Phenotypes | Let |
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| RNAi Reagent Name & Type | sjj_M7.1 (PCR reagent) | ||||||||
| Delivery Method | Bacterial_feeding | ||||||||
| Laboratory | KP | ||||||||
| Date | 2005-5-09 | ||||||||
| Reference | Sieburth D et al. (2005) Nature "Systematic analysis of genes required for synapse structure and ...." | ||||||||
| Gene Plot of M7.1 | |||||||||
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