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Gene/ORF R09B3.4 (ubc-12) Ace View of R09B3.4
R09B3.4 Details Help
Description  The ubc-12 gene encodes a ubiquitin-conjugating enzyme whose substrate is NED-8, and which is required for both embryogenesis and terminal hypodermal differentiation; ubc-12(RNAi) mutants either arrest during embryonic development or show pleotropic defects (e.g., vulval eversion during the L4 stage), as well as defective alae.
Homology Group (KOG)  Ubiquitin-protein ligase
GO Terms  ubiquitin-like activating enzyme activity (GO:0008642) ; protein modification (GO:0006464) ; ubiquitin cycle (GO:0006512)
Location   I:11910915..11912437
RNAi Phenotypes
Early_embryo Cyk
Postembryonic Adl
Larva Gro
Adult Sck ; Pvl ; Pvl ; Unc ; Rup ; Stp ; Stp
External Links  wormbase.org NCBI.org NBrowse
Associated RNAi Experiments View Summary
Cenix:88-g1
Embryonic Phenotypes  Emb Penetrance None reported
Adult Phenotypes  Cyk
RNAi Reagent Name & Type Cenix:88-g1 (PCR reagent)
Delivery Method  Injection
Remark  early embryonic: Cytokinesis
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
JA:R09B3.4
Embryonic Phenotypes  Emb Penetrance 90 %
Adult Phenotypes  Rup
Sck
Adult Phenotypes  Oth <200 cells
RNAi Reagent Name & Type sjj_R09B3.4 (PCR reagent)
Remark  no pronuclear migration
Laboratory  JA
Date  2000-11-16
Reference  Fraser AG et al. (2000) Nature "Functional genomic analysis of C. elegans chromosome I by systematic RNA ...."
Movies/Stacks/Images  /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif
Simmer:R09B3.4:Screen_A
Embryonic Phenotypes  Emb Penetrance 20 - 40 %
Larval Phenotypes  Gro
Adult Phenotypes  Pvl
Stp
Adult Phenotypes  Unclassified Thin
RNAi Reagent Name & Type sjj_R09B3.4 (PCR reagent)
Laboratory  NL
Date  2003-10-01
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
Simmer:R09B3.4:Screen_B
Embryonic Phenotypes  Emb Penetrance 50 - 80 %
Adult Phenotypes  Pvl
Stp
Unc
Adult Phenotypes  Adl
RNAi Reagent Name & Type sjj_R09B3.4 (PCR reagent)
Laboratory  NL
Date  2003-10-01
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
Gene Plot of R09B3.4 Browse using GBrowse
Gene Map