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Gene/ORF T01G9.5 (mei-1) Ace View of T01G9.5
T01G9.5 Details Help
Description  mei-1 encodes, by alternative splicing, two slightly different isoforms of an ortholog of the catalytic subunit of katanin (OMIM:606696) that is required for oocyte meiotic spindle formation, for stable association of the meiosis I spindle with the oocyte cortex, and for microtubule severing; MEI-1 exists in a physical complex with MEI-2 at the polar ends of meiotic spindle microtubules and meiotic chromatin; MEI-1 must be inactivated for meiosis to give way to mitosis, and ectopic expression of MEI-1/MEI-2 during mitosis is normally lethal; MEI-1 is bound by the BTB protein MEL-26 (both in vivo and in vitro), which physically links MEI-1 to CUL-3 (an E3 ubiquitin ligase); CUL-3, in turn, promotes MEI-1 proteolysis by the 26S proteasome during the transition from meiosis to mitosis; the MEI-1/MEI-2 complex requires tubules containing the germline-enriched beta tubulin TBB-2 in vivo, but does not require tubules made from TBB-2's (superficially) redundant paralog TBB-1.
GO Terms  female meiosis (GO:0007143) ; meiotic spindle assembly (GO:0000212) ; spindle (GO:0005819) ; spindle pole (GO:0000922) ; chromatin (GO:0000785) ; nucleus (GO:0005634) ; nucleotide binding (GO:0000166) ; nucleoside triphosphatase activity (GO:0017111) ; ATP binding (GO:0005524)
Location   I:8294472..8297287
RNAi Phenotypes
Early_embryo Abnormal pronuclei ; ABa, ABp and EMS divide synchronously ; Multiple maternal pronuclei ; Multiple nuclei in 1, 2, or 4-cell embryos ; Division axis of ABa or ABp aberrant ; Mei ; Multiple nuclei ; Excessive blebbing ; Abnormal size of pronucleus ; Aberrant cell-cell contacts at the four-cell stage ; Polar bodies abnormal ; Polar bodies abnormal ; Passage Through Meiosis defective
External Links  wormbase.org NCBI.org NBrowse
Associated RNAi Experiments View Details
Experiments with Non-Wild Phenotypes
Cenix:11-h4 Emb ; Passage Through Meiosis defective
Simmer:T01G9.5:Screen_A Emb
JA:T01G9.5 Emb ; Mei
Simmer:T01G9.5:Screen_B Emb
PF:GL1_1F2 ABa, ABp and EMS divide synchronously ; Aberrant cell-cell contacts at the four-cell stage ; Abnormal size of pronucleus ; Division axis of ABa or ABp aberrant ; Emb ; Excessive blebbing ; Multiple maternal pronuclei ; Multiple nuclei in 1, 2, or 4-cell embryos ; Polar bodies abnormal
Gene Plot of T01G9.5 Browse using GBrowse
Gene Map