| Ace View of T01G9.5 | |
| T01G9.5 Details | |
| Description | mei-1 encodes, by alternative splicing, two slightly different isoforms of an ortholog of the catalytic subunit of katanin (OMIM:606696) that is required for oocyte meiotic spindle formation, for stable association of the meiosis I spindle with the oocyte cortex, and for microtubule severing; MEI-1 exists in a physical complex with MEI-2 at the polar ends of meiotic spindle microtubules and meiotic chromatin; MEI-1 must be inactivated for meiosis to give way to mitosis, and ectopic expression of MEI-1/MEI-2 during mitosis is normally lethal; MEI-1 is bound by the BTB protein MEL-26 (both in vivo and in vitro), which physically links MEI-1 to CUL-3 (an E3 ubiquitin ligase); CUL-3, in turn, promotes MEI-1 proteolysis by the 26S proteasome during the transition from meiosis to mitosis; the MEI-1/MEI-2 complex requires tubules containing the germline-enriched beta tubulin TBB-2 in vivo, but does not require tubules made from TBB-2's (superficially) redundant paralog TBB-1. |
| GO Terms | female meiosis (GO:0007143) ; meiotic spindle assembly (GO:0000212) ; spindle (GO:0005819) ; spindle pole (GO:0000922) ; chromatin (GO:0000785) ; nucleus (GO:0005634) ; nucleotide binding (GO:0000166) ; nucleoside triphosphatase activity (GO:0017111) ; ATP binding (GO:0005524) |
| Location | I:8294472..8297287 |
| RNAi Phenotypes | |
| External Links |    |
| Associated RNAi Experiments | |
| Cenix:11-h4 | |
| Embryonic Phenotypes | Emb Penetrance None reported |
| Adult Phenotypes | Passage Through Meiosis defective |
| RNAi Reagent Name & Type | Cenix:11-h4 (PCR reagent) |
| Delivery Method | Injection |
| Remark | early embryonic: Polar Body Extrusion |
| Laboratory | TH |
| Date | 2005-3-24 |
| Reference | Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...." |
| JA:T01G9.5 | |
| Embryonic Phenotypes | Emb Penetrance 100 % |
| Adult Phenotypes | Mei
Differentiated, not enclosed |
| RNAi Reagent Name & Type | sjj_T01G9.5 (PCR reagent) |
| Remark | multiple female pronuclei |
| Laboratory | JA |
| Date | 2000-11-16 |
| Reference | Fraser AG et al. (2000) Nature "Functional genomic analysis of C. elegans chromosome I by systematic RNA ...." | Movies/Stacks/Images |  |
| PF:GL1_1F2 | |
| Embryonic Phenotypes | Emb Penetrance 100 % |
| Adult Phenotypes | ABa, ABp and EMS divide synchronously Aberrant cell-cell contacts at the four-cell stage Abnormal size of pronucleus Division axis of ABa or ABp aberrant Excessive blebbing Multiple maternal pronuclei Multiple nuclei in 1, 2, or 4-cell embryos Polar bodies abnormal |
| RNAi Reagent Name & Type | sjj_T01G9.5 (PCR reagent) |
| Delivery Method | Injection |
| Remark | PCR product contains flanking T7 promoter sequences |
| Laboratory | KK |
| Date | 2002-11-19 |
| Reference | Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans" | Movies/Stacks/Images | |
| Simmer:T01G9.5:Screen_A | |
| Embryonic Phenotypes | Emb Penetrance 100 % |
| RNAi Reagent Name & Type | sjj_T01G9.5 (PCR reagent) |
| Laboratory | NL |
| Date | 2003-10-01 |
| Strain | NL4256 rrf-3(pk1426) |
| Reference | Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...." |
| Simmer:T01G9.5:Screen_B | |
| Embryonic Phenotypes | Emb Penetrance 100 % |
| RNAi Reagent Name & Type | sjj_T01G9.5 (PCR reagent) |
| Laboratory | NL |
| Date | 2003-10-01 |
| Strain | NL4256 rrf-3(pk1426) |
| Reference | Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...." |
| Gene Plot of T01G9.5 | |
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