Gene/ORF
T04A8.14 (emb-5)
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Ace View of T04A8.14
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T04A8.14 Details |
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Description |
emb-5 encodes the C. elegans ortholog of the Spt6 family of RNA polymerase II transcription elongation factors; first identified in screens for temperature-sensitive embryonic lethal mutations, emb-5 is required for the correct timing of the second E (endoderm)-cell division in the early embryo and thus, for normal embryonic gastrulation; in addition, emb-5 activity is required postembryonically for proper gonad development; in yeast two-hybrid studies, EMB-5 interacts with the intracellular domains of LIN-12 and GLP-1, suggesting that EMB-5 functions as a positive downstream effector in Notch-like signaling pathways during C. elegans development; emb-5 mRNA is most abundant during embryonic stages, with lower levels apparent during the L1-L3 larval stages, and even lower levels observed during L4. |
Homology Group (KOG) |
Transcription elongation factor SPT6
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GO Terms |
RNA binding
(GO:0003723) ; intracellular signaling cascade
(GO:0007242) ; hydrolase activity, acting on ester bonds
(GO:0016788) ; nucleobase, nucleoside, nucleotide and nucleic acid metabolism
(GO:0006139) |
Location |
III:4714855..4721027 |
RNAi Phenotypes |
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External Links |
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Associated RNAi Experiments |
View Summary
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Cenix:300-a9
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Adult Phenotypes |
WT
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RNAi Reagent Name & Type |
Cenix:300-a9 (PCR reagent) |
Delivery Method |
Injection |
Remark |
early embryonic: No significant defects. |
Laboratory |
TH |
Date |
2005-3-24 |
Reference |
Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...." |
JA:T04A8.14
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Embryonic Phenotypes |
Emb Penetrance 80 % |
Adult Phenotypes |
Sck
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RNAi Reagent Name & Type |
sjj_T04A8.14 (PCR reagent) |
Laboratory |
JA |
Date |
2003-1-10 |
Reference |
Kamath RS et al. (2003) Nature "Systemic functional analysis of the Caenorhabditis elegans genome using ...." |
TH:314G1
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Adult Phenotypes |
WTDIC phenotype -- Embryonic development normal
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RNAi Reagent Name & Type |
TH:314G1 (PCR reagent) |
Delivery Method |
Injection |
Laboratory |
TH |
Date |
2000-11-16 |
Reference |
Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...." |
Cenix:314-g1
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Embryonic Phenotypes |
Emb Penetrance None reported |
Adult Phenotypes |
WT_postembryonic_morphology
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RNAi Reagent Name & Type |
Cenix:314-g1 (PCR reagent) |
Delivery Method |
Injection |
Laboratory |
TH |
Date |
2005-3-24 |
Reference |
Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...." |
Gene Plot of T04A8.14 |
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