Ace View of T20B12.2 | |||||
T20B12.2 Details | |||||
Description | tbp-1 encodes the C. elegans ortholog of the human TATA-box-binding protein (TBP; OMIM:600075; mutated in spinocerebellar ataxia 17); consistent with its proposed role in transcriptional regulation, TBP-1 has been shown to provide TFIID-like basal transcription activity in human and C. elegans extracts, to bind specifically to a TATA box sequence, and to interact with TFIIA and TFIIB transcription factors from other species; large-scale RNA-mediated interference (RNAi) screens indicate that tbp-1 activity is required for embryonic and larval development, as well as for normal rates of postembryonic growth. | ||||
Homology Group (KOG) | TATA-box binding protein (TBP), component of TFIID and TFIIIB |
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GO Terms | binding (GO:0005488) ; DNA binding (GO:0003677) ; RNA polymerase II transcription factor activity (GO:0003702) ; regulation of transcription, DNA-dependent (GO:0006355) ; transcription initiation from Pol II promoter (GO:0006367) ; nucleus (GO:0005634) ; transcription factor TFIID complex (GO:0005669) | ||||
Location | III:7383551..7386452 | ||||
RNAi Phenotypes |
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External Links | |||||
Associated RNAi Experiments | |||||
Cenix:340-h4 | |||||
Embryonic Phenotypes | Emb Penetrance None reported | ||||
RNAi Reagent Name & Type | Cenix:340-h4 (PCR reagent) | ||||
Delivery Method | Injection | ||||
Laboratory | TH | ||||
Date | 2005-3-24 | ||||
Reference | Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...." | ||||
JA:T20B12.2 | |||||
Embryonic Phenotypes | Emb Penetrance 80 % | ||||
Larval Phenotypes | Gro |
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RNAi Reagent Name & Type | sjj_T20B12.2 (PCR reagent) | ||||
Laboratory | JA | ||||
Date | 2003-1-10 | ||||
Reference | Kamath RS et al. (2003) Nature "Systemic functional analysis of the Caenorhabditis elegans genome using ...." | ||||
Simmer:T20B12.2 | |||||
Larval Phenotypes | Lva |
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RNAi Reagent Name & Type | sjj_T20B12.2 (PCR reagent) | ||||
Laboratory | NL | ||||
Date | 2003-10-01 | ||||
Strain | NL4256 rrf-3(pk1426) | ||||
Reference | Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...." | ||||
TH:302C7 | |||||
Embryonic Phenotypes | Emb Penetrance None reported | ||||
RNAi Reagent Name & Type | TH:302C7 (PCR reagent) | ||||
Remark | Phenotype comment -- A second independent RNA (RNA2) yielded a weaker phenotype (early larval arrest). | ||||
Laboratory | TH | ||||
Date | 2000-11-16 | ||||
Reference | Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...." | ||||
TH:340H4 | |||||
Embryonic Phenotypes | Emb Penetrance None reported | ||||
RNAi Reagent Name & Type | TH:340H4 (PCR reagent) | ||||
Remark | Phenotype comment -- A second independent RNA (RNA2) yielded a weaker phenotype (early larval arrest). | ||||
Laboratory | TH | ||||
Date | 2000-11-16 | ||||
Reference | Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...." | ||||
Cenix:302-c7 | |||||
Adult Phenotypes | Larval Lethal-Early (L1/L2) |
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RNAi Reagent Name & Type | Cenix:302-c7 (PCR reagent) | ||||
Delivery Method | Injection | ||||
Remark | early embryonic: No significant defects. | ||||
Laboratory | TH | ||||
Date | 2005-3-24 | ||||
Reference | Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...." | ||||
Gene Plot of T20B12.2 | |||||