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Gene/ORF T20B12.2 (tbp-1) Ace View of T20B12.2
T20B12.2 Details Help
Description  tbp-1 encodes the C. elegans ortholog of the human TATA-box-binding protein (TBP; OMIM:600075; mutated in spinocerebellar ataxia 17); consistent with its proposed role in transcriptional regulation, TBP-1 has been shown to provide TFIID-like basal transcription activity in human and C. elegans extracts, to bind specifically to a TATA box sequence, and to interact with TFIIA and TFIIB transcription factors from other species; large-scale RNA-mediated interference (RNAi) screens indicate that tbp-1 activity is required for embryonic and larval development, as well as for normal rates of postembryonic growth.
Homology Group (KOG)  TATA-box binding protein (TBP), component of TFIID and TFIIIB
GO Terms  binding (GO:0005488) ; DNA binding (GO:0003677) ; RNA polymerase II transcription factor activity (GO:0003702) ; regulation of transcription, DNA-dependent (GO:0006355) ; transcription initiation from Pol II promoter (GO:0006367) ; nucleus (GO:0005634) ; transcription factor TFIID complex (GO:0005669)
Location   III:7383551..7386452
RNAi Phenotypes
Postembryonic Larval Lethal-Early (L1/L2)
Larva Lva ; Gro
External Links  wormbase.org NCBI.org NBrowse
Associated RNAi Experiments View Summary
Cenix:340-h4
Embryonic Phenotypes  Emb Penetrance None reported
RNAi Reagent Name & Type Cenix:340-h4 (PCR reagent)
Delivery Method  Injection
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
JA:T20B12.2
Embryonic Phenotypes  Emb Penetrance 80 %
Larval Phenotypes  Gro
RNAi Reagent Name & Type sjj_T20B12.2 (PCR reagent)
Laboratory  JA
Date  2003-1-10
Reference  Kamath RS et al. (2003) Nature "Systemic functional analysis of the Caenorhabditis elegans genome using ...."
Simmer:T20B12.2
Larval Phenotypes  Lva
RNAi Reagent Name & Type sjj_T20B12.2 (PCR reagent)
Laboratory  NL
Date  2003-10-01
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
TH:302C7
Embryonic Phenotypes  Emb Penetrance None reported
RNAi Reagent Name & Type TH:302C7 (PCR reagent)
Remark  Phenotype comment -- A second independent RNA (RNA2) yielded a weaker phenotype (early larval arrest).
Laboratory  TH
Date  2000-11-16
Reference  Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...."
TH:340H4
Embryonic Phenotypes  Emb Penetrance None reported
RNAi Reagent Name & Type TH:340H4 (PCR reagent)
Remark  Phenotype comment -- A second independent RNA (RNA2) yielded a weaker phenotype (early larval arrest).
Laboratory  TH
Date  2000-11-16
Reference  Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...."
Cenix:302-c7
Adult Phenotypes  Larval Lethal-Early (L1/L2)
RNAi Reagent Name & Type Cenix:302-c7 (PCR reagent)
Delivery Method  Injection
Remark  early embryonic: No significant defects.
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
Gene Plot of T20B12.2 Browse using GBrowse
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