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Gene/ORF W02D9.1 (pri-2) Ace View of W02D9.1
W02D9.1 Details Help
Description  pri-2 encodes a homolog of the DNA polymerase alpha-primase subunit C that is required in embryos for the normal timing of embryonic cell divisions (much as DIV-1 is) and for normal mitosis in the germline.
Homology Group (KOG)  Eukaryotic-type DNA primase, large subunit
GO Terms  DNA primase activity (GO:0003896) ; DNA replication, priming (GO:0006269) ; alpha DNA polymerase:primase complex (GO:0005658)
Location   I:12527904..12532569
RNAi Phenotypes
Early_embryo Exaggerated asynchrony ; Aberrant AB or P1 spindle orientation ; Multiple cavities ; Multiple nuclei in 1, 2, or 4-cell embryos ; Ectopic cleavage furrows ; Excessive blebbing ; Slow cell cycle ; Polar body reabsorbed ; Aberrant cell-cell contacts at the four-cell stage ; Nmo
Adult Pvl ; Pvl ; Ste
External Links  wormbase.org NCBI.org NBrowse
Associated RNAi Experiments View Summary
Cenix:143-c7
Embryonic Phenotypes  Emb Penetrance None reported
RNAi Reagent Name & Type Cenix:143-c7 (PCR reagent)
Delivery Method  Injection
Remark  early embryonic: Reproducible phenotype. Significant drop in the overall pace of events and delayed P-lineage division. Chromatin segregation defect at the four cell stage in some embryos.
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
JA:W02D9.1
Embryonic Phenotypes  Emb Penetrance 100 %
Adult Phenotypes  Pvl
Adult Phenotypes  Nmo <200 cells
RNAi Reagent Name & Type sjj_W02D9.1 (PCR reagent)
Remark  P1 divides too late
Laboratory  JA
Date  2000-11-16
Reference  Fraser AG et al. (2000) Nature "Functional genomic analysis of C. elegans chromosome I by systematic RNA ...."
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PF:GL1_2B12
Embryonic Phenotypes  Emb Penetrance 100 %
Adult Phenotypes  Aberrant AB or P1 spindle orientation
Aberrant cell-cell contacts at the four-cell stage
Ectopic cleavage furrows
Exaggerated asynchrony
Excessive blebbing
Multiple cavities
Multiple nuclei in 1, 2, or 4-cell embryos
Polar body reabsorbed
Slow cell cycle
RNAi Reagent Name & Type sjj_W02D9.1 (PCR reagent)
Delivery Method  Injection
Remark  PCR product contains flanking T7 promoter sequences
Laboratory  KK
Date  2002-11-19
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
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Simmer:W02D9.1:Screen_A
Embryonic Phenotypes  Emb Penetrance 100 %
RNAi Reagent Name & Type sjj_W02D9.1 (PCR reagent)
Laboratory  NL
Date  2003-10-01
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
Simmer:W02D9.1:Screen_B
Embryonic Phenotypes  Emb Penetrance 100 %
RNAi Reagent Name & Type sjj_W02D9.1 (PCR reagent)
Laboratory  NL
Date  2003-10-01
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
Gene Plot of W02D9.1 Browse using GBrowse
Gene Map