Gene/ORF
ZC404.8 (spn-4)
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Ace View of ZC404.8
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ZC404.8 Details |
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Description |
spn-4 encodes a protein containing an RNP-type RNA-binding domain; SPN-4 is required for rotation, but not polarization, of the mitotic spindle in the P1 blastomere of the two-cell stage embryo; SPN-4 is also required for mesectoderm and mesendoderm formation in conjunction with proper localization of maternal cell-fate determinants such as SKN-1; in addition, SPN-4 binds the glp-1 3' UTR and is necessary for glp-1 mRNA translation in the anterior blastomeres of the early embryo; conversely, SPN-4 binds and suppresses translation of pal-1 and skn-1 mRNAs in anterior blastomeres; in vitro, SPN-4 interacts with POS-1, a CCCH-type zinc finger protein that negatively regulates glp-1 mRNA translation in posterior blastomeres; SPN-4 is a P granule component, but is also detected in oocytes, the AB and P1 blastomeres at the two-cell stage, and in all four blastomeres at the four-cell stage, with higher levels in EMS and P2. |
Homology Group (KOG) |
Ataxin 2-binding protein (RRM superfamily)
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GO Terms |
nucleotide binding
(GO:0000166) ; nucleic acid binding
(GO:0003676) |
Location |
V:6785380..6787897 |
RNAi Phenotypes |
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External Links |
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Associated RNAi Experiments |
View Details
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Cenix:28-f1
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Embryonic Phenotypes |
Emb Penetrance None reported |
Adult Phenotypes |
Complex Phenotype
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RNAi Reagent Name & Type |
Cenix:28-f1 (PCR reagent) |
Delivery Method |
Injection |
Remark |
early embryonic: Complex Phenotype |
Laboratory |
TH |
Date |
2005-3-24 |
Reference |
Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...." |
JA:ZC404.8
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Adult Phenotypes |
WT
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RNAi Reagent Name & Type |
sjj_ZC404.8 (PCR reagent) |
Laboratory |
JA |
Date |
2003-1-10 |
Reference |
Kamath RS et al. (2003) Nature "Systemic functional analysis of the Caenorhabditis elegans genome using ...." |
Gene Plot of ZC404.8 |
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