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Gene/ORF ZC404.8 (spn-4) Ace View of ZC404.8
ZC404.8 Details Help
Description  spn-4 encodes a protein containing an RNP-type RNA-binding domain; SPN-4 is required for rotation, but not polarization, of the mitotic spindle in the P1 blastomere of the two-cell stage embryo; SPN-4 is also required for mesectoderm and mesendoderm formation in conjunction with proper localization of maternal cell-fate determinants such as SKN-1; in addition, SPN-4 binds the glp-1 3' UTR and is necessary for glp-1 mRNA translation in the anterior blastomeres of the early embryo; conversely, SPN-4 binds and suppresses translation of pal-1 and skn-1 mRNAs in anterior blastomeres; in vitro, SPN-4 interacts with POS-1, a CCCH-type zinc finger protein that negatively regulates glp-1 mRNA translation in posterior blastomeres; SPN-4 is a P granule component, but is also detected in oocytes, the AB and P1 blastomeres at the two-cell stage, and in all four blastomeres at the four-cell stage, with higher levels in EMS and P2.
Homology Group (KOG)  Ataxin 2-binding protein (RRM superfamily)
GO Terms  nucleotide binding (GO:0000166) ; nucleic acid binding (GO:0003676)
Location   V:6785380..6787897
RNAi Phenotypes
Early_embryo Complex Phenotype
External Links  wormbase.org NCBI.org NBrowse
Associated RNAi Experiments View Details
Cenix:28-f1
Embryonic Phenotypes  Emb Penetrance None reported
Adult Phenotypes  Complex Phenotype
RNAi Reagent Name & Type Cenix:28-f1 (PCR reagent)
Delivery Method  Injection
Remark  early embryonic: Complex Phenotype
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
JA:ZC404.8
Adult Phenotypes  WT
RNAi Reagent Name & Type sjj_ZC404.8 (PCR reagent)
Laboratory  JA
Date  2003-1-10
Reference  Kamath RS et al. (2003) Nature "Systemic functional analysis of the Caenorhabditis elegans genome using ...."
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