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Gene/ORF ZK1151.1 (vab-10) Ace View of ZK1151.1
ZK1151.1 Details Help
Description  vab-10 encodes, by alternative splicing, two spectraplakins (VAB-10A and VAB-10B) that are jointly required for mechanical resilience of the epidermis under strain by the contraction of actin microfilaments mechanically linked to fibrous organelles (FOs); VAB-10A/B are, accordingly, required for elongation during embryonic morphogenesis; VAB-10A has C-terminal plectin repeats while VAB-10B has spectrin repeats; VAB-10A is required to keep the epidermis and extracellular matrix attached via FOs, while VAB-10B is required to keep the apical and basal epidermal plasma membranes connected during morphogenesis; vab-10a and vab-10b mutations complement one another; vab-10 is orthologous to human BPAG1 (dystonin or MACF2, OMIM:113810, encoding an autoantigen of bullous pemphigoid blistering disease) and dJ562N20.1 (MACF1), and to Drosophila SHORT STOP.
Homology Group (KOG)  Dystonin, GAS (Growth-arrest-specific protein), and related proteins
GO Terms  actin binding (GO:0003779) ; calcium ion binding (GO:0005509) ; cell cycle arrest (GO:0007050)
Location   I:11747016..11792506
RNAi Phenotypes
Postembryonic Prz ; Prz ; WT_postembryonic_morphology ; WT_postembryonic_morphology
Larva Let ; Lva ; Lva ; Lvl ; Lvl ; Lvl ; Gro
Adult Bmd ; Bmd ; Bmd ; Pvl ; Pvl ; Unc ; Unc ; Unc ; Ste ; Rup ; Rup ; Rup ; Stp
External Links  wormbase.org NCBI.org NBrowse
Associated RNAi Experiments View Summary
Cenix:155-d9
Adult Phenotypes  WT
RNAi Reagent Name & Type Cenix:155-d9 (PCR reagent)
Delivery Method  Injection
Remark  Wildtype results in all three tests.
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
Cenix:20-f11
RNAi Reagent Name & Type Cenix:20-f11 (PCR reagent)
Delivery Method  Injection
Remark  early embryonic: Wildtype results in two independent experiments.
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
JA:ZK1151.1
Adult Phenotypes  WT
RNAi Reagent Name & Type sjj_ZK1151.1 (PCR reagent)
Laboratory  JA
Date  2003-1-10
Reference  Kamath RS et al. (2003) Nature "Systemic functional analysis of the Caenorhabditis elegans genome using ...."
JA:ZK1151.2
Adult Phenotypes  Bmd
Stp
Unc
RNAi Reagent Name & Type sjj_ZK1151.2 (PCR reagent)
Laboratory  JA
Date  2000-11-16
Reference  Fraser AG et al. (2000) Nature "Functional genomic analysis of C. elegans chromosome I by systematic RNA ...."
Simmer:ZK1151.1:Repeat_b
Larval Phenotypes  Lva
Lvl
Adult Phenotypes  Pvl
Rup
Adult Phenotypes  Prz
RNAi Reagent Name & Type sjj_ZK1151.1 (PCR reagent)
Laboratory  NL
Date  2003-10-01
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
Simmer:ZK1151.1:Screen_A
Adult Phenotypes  Pvl
Rup
Ste
Adult Phenotypes  Prz
RNAi Reagent Name & Type sjj_ZK1151.1 (PCR reagent)
Laboratory  NL
Date  2003-10-01
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
Simmer:ZK1151.1:Screen_B
Embryonic Phenotypes  Emb Penetrance 100 %
RNAi Reagent Name & Type sjj_ZK1151.1 (PCR reagent)
Laboratory  NL
Date  2003-10-01
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
Simmer:ZK1151.2:Screen_A
Larval Phenotypes  Gro
Lvl
Adult Phenotypes  Bmd
Rup
Unc
RNAi Reagent Name & Type sjj_ZK1151.2 (PCR reagent)
Laboratory  NL
Date  2003-10-01
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
Simmer:ZK1151.2:Screen_B
Larval Phenotypes  Lva
Lvl
Adult Phenotypes  Bmd
Unc
RNAi Reagent Name & Type sjj_ZK1151.2 (PCR reagent)
Laboratory  NL
Date  2003-10-01
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
Cenix:29-e9
Embryonic Phenotypes  Emb Penetrance None reported
Adult Phenotypes  WT_postembryonic_morphology
RNAi Reagent Name & Type Cenix:29-e9 (PCR reagent)
Delivery Method  Injection
Remark  early embryonic: No reproducible defects found.
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
Cenix:63-f12
Embryonic Phenotypes  Emb Penetrance None reported
Adult Phenotypes  WT_postembryonic_morphology
RNAi Reagent Name & Type Cenix:63-f12 (PCR reagent)
Delivery Method  Injection
Remark  70-100% embryonic lethality. Most escapers die at larval stage.
Laboratory  TH
Date  2005-3-24
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
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