Ace View of ZK1151.1 | |||||||
ZK1151.1 Details | |||||||
Description | vab-10 encodes, by alternative splicing, two spectraplakins (VAB-10A and VAB-10B) that are jointly required for mechanical resilience of the epidermis under strain by the contraction of actin microfilaments mechanically linked to fibrous organelles (FOs); VAB-10A/B are, accordingly, required for elongation during embryonic morphogenesis; VAB-10A has C-terminal plectin repeats while VAB-10B has spectrin repeats; VAB-10A is required to keep the epidermis and extracellular matrix attached via FOs, while VAB-10B is required to keep the apical and basal epidermal plasma membranes connected during morphogenesis; vab-10a and vab-10b mutations complement one another; vab-10 is orthologous to human BPAG1 (dystonin or MACF2, OMIM:113810, encoding an autoantigen of bullous pemphigoid blistering disease) and dJ562N20.1 (MACF1), and to Drosophila SHORT STOP. | ||||||
Homology Group (KOG) | Dystonin, GAS (Growth-arrest-specific protein), and related proteins |
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GO Terms | actin binding (GO:0003779) ; calcium ion binding (GO:0005509) ; cell cycle arrest (GO:0007050) | ||||||
Location | I:11747016..11792506 | ||||||
RNAi Phenotypes |
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External Links | |||||||
Associated RNAi Experiments | |||||||
Cenix:155-d9 | |||||||
Adult Phenotypes | WT |
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RNAi Reagent Name & Type | Cenix:155-d9 (PCR reagent) | ||||||
Delivery Method | Injection | ||||||
Remark | Wildtype results in all three tests. | ||||||
Laboratory | TH | ||||||
Date | 2005-3-24 | ||||||
Reference | Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...." | ||||||
Cenix:20-f11 | |||||||
RNAi Reagent Name & Type | Cenix:20-f11 (PCR reagent) | ||||||
Delivery Method | Injection | ||||||
Remark | early embryonic: Wildtype results in two independent experiments. | ||||||
Laboratory | TH | ||||||
Date | 2005-3-24 | ||||||
Reference | Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...." | ||||||
JA:ZK1151.1 | |||||||
Adult Phenotypes | WT |
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RNAi Reagent Name & Type | sjj_ZK1151.1 (PCR reagent) | ||||||
Laboratory | JA | ||||||
Date | 2003-1-10 | ||||||
Reference | Kamath RS et al. (2003) Nature "Systemic functional analysis of the Caenorhabditis elegans genome using ...." | ||||||
JA:ZK1151.2 | |||||||
Adult Phenotypes | Bmd Stp Unc |
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RNAi Reagent Name & Type | sjj_ZK1151.2 (PCR reagent) | ||||||
Laboratory | JA | ||||||
Date | 2000-11-16 | ||||||
Reference | Fraser AG et al. (2000) Nature "Functional genomic analysis of C. elegans chromosome I by systematic RNA ...." | ||||||
Simmer:ZK1151.1:Repeat_b | |||||||
Larval Phenotypes | Lva Lvl |
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Adult Phenotypes | Pvl Rup |
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Adult Phenotypes | Prz |
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RNAi Reagent Name & Type | sjj_ZK1151.1 (PCR reagent) | ||||||
Laboratory | NL | ||||||
Date | 2003-10-01 | ||||||
Strain | NL4256 rrf-3(pk1426) | ||||||
Reference | Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...." | ||||||
Simmer:ZK1151.1:Screen_A | |||||||
Adult Phenotypes | Pvl Rup Ste |
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Adult Phenotypes | Prz |
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RNAi Reagent Name & Type | sjj_ZK1151.1 (PCR reagent) | ||||||
Laboratory | NL | ||||||
Date | 2003-10-01 | ||||||
Strain | NL4256 rrf-3(pk1426) | ||||||
Reference | Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...." | ||||||
Simmer:ZK1151.1:Screen_B | |||||||
Embryonic Phenotypes | Emb Penetrance 100 % | ||||||
RNAi Reagent Name & Type | sjj_ZK1151.1 (PCR reagent) | ||||||
Laboratory | NL | ||||||
Date | 2003-10-01 | ||||||
Strain | NL4256 rrf-3(pk1426) | ||||||
Reference | Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...." | ||||||
Simmer:ZK1151.2:Screen_A | |||||||
Larval Phenotypes | Gro Lvl |
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Adult Phenotypes | Bmd Rup Unc |
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RNAi Reagent Name & Type | sjj_ZK1151.2 (PCR reagent) | ||||||
Laboratory | NL | ||||||
Date | 2003-10-01 | ||||||
Strain | NL4256 rrf-3(pk1426) | ||||||
Reference | Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...." | ||||||
Simmer:ZK1151.2:Screen_B | |||||||
Larval Phenotypes | Lva Lvl |
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Adult Phenotypes | Bmd Unc |
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RNAi Reagent Name & Type | sjj_ZK1151.2 (PCR reagent) | ||||||
Laboratory | NL | ||||||
Date | 2003-10-01 | ||||||
Strain | NL4256 rrf-3(pk1426) | ||||||
Reference | Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...." | ||||||
Cenix:29-e9 | |||||||
Embryonic Phenotypes | Emb Penetrance None reported | ||||||
Adult Phenotypes | WT_postembryonic_morphology |
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RNAi Reagent Name & Type | Cenix:29-e9 (PCR reagent) | ||||||
Delivery Method | Injection | ||||||
Remark | early embryonic: No reproducible defects found. | ||||||
Laboratory | TH | ||||||
Date | 2005-3-24 | ||||||
Reference | Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...." | ||||||
Cenix:63-f12 | |||||||
Embryonic Phenotypes | Emb Penetrance None reported | ||||||
Adult Phenotypes | WT_postembryonic_morphology |
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RNAi Reagent Name & Type | Cenix:63-f12 (PCR reagent) | ||||||
Delivery Method | Injection | ||||||
Remark | 70-100% embryonic lethality. Most escapers die at larval stage. | ||||||
Laboratory | TH | ||||||
Date | 2005-3-24 | ||||||
Reference | Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...." | ||||||
Gene Plot of ZK1151.1 | |||||||