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Go to RNAiDB Home Adult Wild-type C. elegans Movie Wild-type C. elegans Movie Wild-type C. elegans Movie Wild-type C. elegans Movie
 
RNAi Experiment: [cgc3752]:cdk-4 Ace View of [cgc3752]:cdk-4
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  F19B2.4 (srz-36)
Evidence for Inhibition 
Sliding Window Raw score: 92
Relative score: 0.093 (Best raw score= for Gene )
Specificity index: 0.066
External "F19B2.4" Links  Wormbase NCBI
Gene Inhibited  F18H3.5 (cdk-4)
Description of "F18H3.5"  cdk-4 encodes two isoforms of a cyclin-dependent serine/threonine protein kinase orthologous to human CDK4 and CDK6 (OMIM:123829 and OMIM:603368, mutated in cutanous malignant melanoma) which complex with D-type cyclins to regulate progression through the G1 phase of the cell cycle; CDK-4 activity is essential for G1 progression in postembryonic blast cells and as a result, cdk-4 mutant animals generally arrest during larval stages; the lethality generated by cdk mutations, also seen in animals doubly mutant for cdk-4 and cyd-1, a C. elegans D-type cyclin, can be suppressed by mutations in lin-35/Rb suggesting that, as in other organisms, LIN-35/Rb may be a major target of CDK-4/CYD-1 kinase activity; CDK-4 expression is first detected in neuronal and hypodermal lineages during mid-to-late embryogenesis, with postembryonic expression detected in hypodermal seam cells, cells of the P lineage which will give rise to ventral cord neurons, and cells of the somatic gonad, the vulva, and the intestine.
KOG Annotation  Protein kinase PCTAIRE and related kinases
What's a KOG?
Evidence for Inhibition 
Sliding Window Raw score: 990
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.706
Wormbase (BLAST) RNAi primary
External "F18H3.5" Links  Wormbase NCBI
Gene Inhibited  F41H8.3 (srx-30)
Evidence for Inhibition 
Sliding Window Raw score: 320
Relative score: 0.323 (Best raw score= for Gene )
Specificity index: 0.228
External "F41H8.3" Links  Wormbase NCBI
[cgc3752]:cdk-4 Phenotypes Help with Phenotypes
Larval  Lvl Low penetrance L2 arrest
Adult  Pvl
Unc
[cgc3752]:cdk-4 Experiment Details Ace View
Laboratory  KM
RNAi Reagent & Type  yk76f3 (cDNA_reagent)
Remark  defects are due to problems in cell proliferation: post-embryonic somatic blast cells sometimes fail to divide, including P blast cells, mesoblast M, somatic gonad precursor Z1 and Z4, and the intestinal nuclei.
Reference  Park M et al. (1999) Development "Regulation of postembryonic G1 cell cycle progression in Caenorhabditis ...."
Delivery Method  Injection
Date  1999-10-06
Gene Plot of F19B2.4 Browse using GBrowse
Gene Graph
Gene Plot of F18H3.5 Browse using GBrowse
Gene Graph
Gene Plot of F41H8.3 Browse using GBrowse
Gene Graph