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RNAi Experiment: [cgc5264]:sep-1 Ace View of [cgc5264]:sep-1
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  Y47G6A.12 (sep-1)
Description of "Y47G6A.12"  The sep-1 gene encodes a homolog of separase, a protease that splits segregating chromosomes at their centromeres during anaphase by proteolyzing the Scc1 subunit of cohesin.
KOG Annotation  Regulator of spindle pole body duplication
What's a KOG?
Evidence for Inhibition 
Sliding Window Raw score: 453
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "Y47G6A.12" Links  Wormbase NCBI
[cgc5264]:sep-1 Phenotypes Help with Phenotypes
Phenotype  Mei In embryos depleted of both GLC-7 and SEP-1 activity, during the first meiotic division, chromatid separation was greatly inhibited; the chromatin became somewhat dispersed, and individual chromosomes could occasionally be observed, but they never fully separate from the reainder of the chromatin as is the case when GLC-7 alone is depleted. glc-7alpha and glc-7beta were simultaneously depleted as well.
[cgc5264]:sep-1 Experiment Details Ace View
Laboratory  MG
RNAi Reagent & Type  [cgc4997]:sep-1 (PCR_reagent)
Strain  N2
Remark  GFP-histone H2B was used to visualize the chromatin
Reference  Kaitna S et al. (2002) Current Biology "The aurora B kinase AIR-2 regulates kinetochores during mitosis and is required ...."
Date  2002-4-03
Gene Plot of Y47G6A.12 Browse using GBrowse
Gene Graph