Link to M05B5.3Link to M05B5.4RNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentgeneMap Link to M05B5.4Link to M05B5.5a.2Link to M05B5.5a.1RNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentgeneMap
Go to RNAiDB Home Adult Wild-type C. elegans Movie Wild-type C. elegans Movie Wild-type C. elegans Movie Wild-type C. elegans Movie
RNAi Experiment: [cgc5655]:M05B5.4 Ace View of [cgc5655]:M05B5.4
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  M05B5.4
Description of "M05B5.4"  The M05B5.4 gene encodes an ortholog of the human gene LECITHIN-CHOLESTEROL ACYLTRANSFERASE (LCAT; OMIM:606967), which when mutated leads to lecithin-cholesterol acyltransferase deficiency (OMIM:245900).
KOG Annotation  Lecithin:cholesterol acyltransferase (LCAT)/Acyl-ceramide synthase
What's a KOG?
Evidence for Inhibition 
ePCR Status: Best_ePCR (I:7189618..7190753)
Transcript overlap: 728 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 665
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.802
Wormbase (BLAST) RNAi primary
External "M05B5.4" Links  Wormbase NCBI
Gene Inhibited  M05B5.5 (hlh-2)
Description of "M05B5.5"  hlh-2 encodes a Class I basic helix-loop-helix (bHLH) transcription factor that is the C. elegans ortholog of the mammalian E and Drosophila Daughterless transcriptional activators; HLH-2 activity is required for cell fate specifications occuring during embryonic and larval development that affect such processes as gonadogenesis, male tail formation, and programmed cell death; HLH-2 has been shown to dimerize with at least two C. elegans Acheate-scute homologs, LIN-32, a neural-specific protein with which it functions in male tail development and HLH-3, with which it is coexpressed in the nuclei of embryonic neuronal prescursors and with which it regulates the transcription of the EGL-1 cell death activator in the NSM sister cells; in gonadogenesis, HLH-2 is required for bestowing proAC competence on the cells that undergo the AC/VU (anchor cell/ventral uterine precursor) cell fate decision, for specification, differentiation, and function of the distal tip cell (DTC) and AC, including transcriptional regulation of the LAG-2 Delta-like ligand in the latter, and for formation of the uterine seam cell (utse); genetic analysis also suggests that HLH-2 functions with HLH-14, an additional Acaete-scute homolog, to specify the PVQ/HSN/PHB neuroblast cell lineage; HLH-2 is expressed in all nuclei of early embryos until the ~200-cell stage, when expression becomes increasingly restricted to neuronal cells and their immediate precursors; later expression is detected in, but not limited to, pharyngeal cells, anterior neurons, vulval and uterine muscles, the DTCs, the presumptive and mature AC, the Q neuroblast, and enteric muscles; comparative analysis of transcriptional and translational reporters indicates that hlh-2 is expressed in both the anchor cell and the ventral uterine (VU) precursor, but that expression in the latter is subject to post-transcriptional down-regulation; HLH-2 accumulation in the presumptive AC is the first detectable difference between the AC and VU precursors during the lateral specification event that distinguishes these two cell fates.
KOG Annotation  Helix loop helix transcription factor
What's a KOG?
Evidence for Inhibition 
ePCR Status: Bottom50_percentile_ePCR (I:7189618..7190753)
Transcript overlap: 185 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 164
Relative score: 0.247 (Best raw score= for Gene )
Specificity index: 0.198
External "M05B5.5" Links  Wormbase NCBI
[cgc5655]:M05B5.4 Phenotypes Help with Phenotypes
Phenotype  Unclassified lipid metabolism abnormal
[cgc5655]:M05B5.4 Experiment Details Ace View
Laboratory  GR
RNAi Reagent & Type  sjj_M05B5.4 (PCR_reagent)
Reference  Ashrafi K et al. (2003) Nature "Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory ...."
Delivery Method  Bacterial_feeding
Date  2003-1-10
Gene Plot of M05B5.5 Browse using GBrowse
Gene Graph
Gene Plot of M05B5.4 Browse using GBrowse
Gene Graph