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RNAi Experiment: Cenix:186-d3 Ace View of Cenix:186-d3
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  K08E7.3 (let-99)
Description of "K08E7.3"  let-99 encodes a protein with a DEP domain (Domain found in Dishevelled, Egl-10, and Pleckstrin); LET-99 has no obvious homologs in non-nematodes, but has a truncated paralog (LRG-1) in C. elegans; LET-99 is required for the mitotic spindle to be properly oriented with respect to the axis of cellular polarity, both during anterior migration and rotation of the nuclear-centrosome complex and during anaphase; let-99 mutants exhibit hyperactive nuclear movement and abnormal anaphase spindle pole behavior; LET-99 is mislocalized in par-2 and par-3 mutants; like other proteins containing DEP domains, LET-99 may act as part of a G protein signalling pathway (e.g., the one controlling spindle position).
Evidence for Inhibition 
ePCR Status: Unique_ePCR (IV:12568030..12568173)
Transcript overlap: 144 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 123
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "K08E7.3" Links  Wormbase NCBI Phenobank 1
Cenix:186-d3 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance None reported
Phenotype  Pnm
External Movies 
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Cenix:186-d3 Experiment Details Ace View
Laboratory  TH
RNAi Reagent & Type  Cenix:186-d3 (PCR_reagent)
Remark  early embryonic: Pronuclear Migration
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
Delivery Method  Injection
Date  2005-3-24
Gene Plot of K08E7.3 Browse using GBrowse
Gene Graph