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RNAi Experiment: Cenix:186-e7 Ace View of Cenix:186-e7
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  M7.1 (let-70)
Description of "M7.1"  let-70 encodes a class I E2 ubiquitin conjugating enzyme; let-70 was identified in screens for essential genes and phenotypic analyses indicate that loss of let-70 activity results in embryonic and larval lethality with defects in sarcomere assembly; purified LET-70 can stimulate the self-ubiquitylation activities of CHN-1 and UFD-2, E4 ubiquitin conjugation factors and, in addition, can stimulate the CHN-1- and UFD-2-dependent multiubiquitylation of UNC-45, a myosin-directed chaperone; in this manner, LET-70 may regulate UNC-45 degradation and myosin assembly; RNA blot analyses and a let-70-lacZ reporter fusion reveal that let-70 is abundantly expressed at all stages of development, including the dauer larval stage; strong staining is observed in most somatic tissues until adult stages, when expression generally becomes restricted to the nervous system.
KOG Annotation  Ubiquitin-protein ligase
What's a KOG?
Evidence for Inhibition 
ePCR Status: Unique_ePCR (IV:11081780..11081894)
Transcript overlap: 106 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 66
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "M7.1" Links  Wormbase NCBI Phenobank 1
Cenix:186-e7 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance None reported
Phenotype  Complex Phenotype
External Movies 
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Cenix:186-e7 Experiment Details Ace View
Laboratory  TH
RNAi Reagent & Type  Cenix:186-e7 (PCR_reagent)
Remark  early embryonic: Complex Phenotype
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
Delivery Method  Injection
Date  2005-3-24
Gene Plot of M7.1 Browse using GBrowse
Gene Graph