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RNAi Experiment: Cenix:230-c1 Ace View of Cenix:230-c1
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  B0336.8 (lgg-3)
Description of "B0336.8"  lgg-3 encodes a hydrophilic protein that is orthologous to Saccharomyces cerevisiae autophagy protein Apg12p; by homology, LGG-3 is predicted to function as a modifier protein required for a protein conjugation system essential for autophagy; in the context of this conjugation system, LGG-3 is predicted to interact with Apg7p and Apg5p homologs (encoded by M7.5 and Y71G12B.12a, respectively) to effect bulk degradation of cytoplasmic components under conditions of starvation or cellular remodeling; free LGG-3 is predicted to localize to the cytoplasm, while LGG-3 conjugated to the Apg5p homolog is predicted to associate with membrane compartments; loss of lgg-3 activity via large-scale RNAi screens results in no obvious developmental or behavioral abnormalities.
KOG Annotation  Protein conjugation factor involved in autophagy
What's a KOG?
Evidence for Inhibition 
ePCR Status: Unique_ePCR (III:5697786..5697960)
Transcript overlap: 173 nt		 Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 152
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "B0336.8" Links  Wormbase NCBI Phenobank 1
Cenix:230-c1 Phenotypes Help with Phenotypes
Phenotype  WT
Cenix:230-c1 Experiment Details Ace View
Laboratory  TH
RNAi Reagent & Type  Cenix:230-c1 (PCR_reagent)
Remark  Wildtype results in all three tests.
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
Delivery Method  Injection
Date  2005-3-24
Gene Plot of B0336.8 Browse using GBrowse
Gene Graph