RNAi Card Page

RNAi Experiment: Cenix:302-f10

Genes Inhibited

Gene Inhibited

Description of "C14B9.4"

plk-1 encodes a serine/threonine polo-like kinase homologous to Drosophila polo and Saccharomyces cerevisiae CDC5; PLK-1 is required for meiotic nuclear envelope breakdown, polar body formation and extrusion, and proper chromosome segregation during meiosis, and additionally, may play a role in germline development; PLK-1 is proposed to phosphorylate REC-8, a meiotic-specific cohesin, thus promoting REC-8 degradation and subsequent separation of sister chromatids; PLK-1 is localized diffusely in the cytoplasm of mature oocytes, but during meiosis I becomes associated with pre-metaphase chromatin and then at anaphase with chromatin and the area between dividing chromatin; in embryos, PLK-1 localizes to centrosomes, metaphase chromatin, and during telophase, to the area between dividing chromatin.

Evidence for Inhibition

ePCR	Status: Unique_ePCR (III:8102444..8104617) Transcript overlap: 1411 nt
Sliding Window	Raw score: 1327 Relative score: 1 (This gene has the best score for this RNAi reagent) Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST)	RNAi primary