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RNAi Experiment: Cenix:304-e11 Ace View of Cenix:304-e11
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  F31E3.5 (eft-3)
Description of "F31E3.5"  eft-3 encodes a translation elongation factor 1-alpha homolog that is required for embryonic viability, fertility, and germline maintenance; the EFT-3 protein sequence is encoded both by eft-3 (F31E3.5) itself but also by R03G5.1.
KOG Annotation  Translation elongation factor EF-1 alpha/Tu
What's a KOG?
Evidence for Inhibition 
ePCR Status: Unique_ePCR (III:6969731..6971688)
Transcript overlap: 1550 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 1466
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.859
Wormbase (BLAST) RNAi primary
External "F31E3.5" Links  Wormbase NCBI Phenobank 1
Gene Inhibited  R03G5.1 (eft-4)
Description of "R03G5.1"  eft-4 encodes a translation elongation factor 1-alpha homolog that is required for embryonic viability, fertility, and a normal postembryonic growth rate; eft-4 encodes at least one alternatively spliced coding sequence identical to that of eft-3.
Evidence for Inhibition 
Sliding Window Raw score: 329
Relative score: 0.164 (Best raw score= for Gene )
Specificity index: 0.141
Wormbase (BLAST) RNAi secondary
External "R03G5.1" Links  Wormbase NCBI Phenobank 1
Cenix:304-e11 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance None reported
Phenotype  WT_postembryonic_morphology
External Movies 
(Please note that RNAiDB is not
hosting these data.)
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Cenix:304-e11 Experiment Details Ace View
Laboratory  TH
RNAi Reagent & Type  Cenix:304-e11 (PCR_reagent)
Remark  early embryonic: No significant defects.
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
Delivery Method  Injection
Date  2005-3-24
Gene Plot of R03G5.1 Browse using GBrowse
Gene Graph
Gene Plot of F31E3.5 Browse using GBrowse
Gene Graph