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RNAi Experiment: Cenix:307-c1 Ace View of Cenix:307-c1
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  C02F5.1 (knl-1)
Description of "C02F5.1"  knl-1 encodes a novel acidic protein with a coiled-coil region at its C-terminus; KNL-1 is an essential kinetochore component that is required for proper spindle elongation and chromosome separation, and in the kinetochore assembly pathway, plays a key role in linking the initiation of kinetochore formation with the construction of a functional microtubule-binding interface; in the assembly pathway, KNL-1 functions downstream of the DNA-proximal kinetochore components CeCENP-A/HCP-3 and CeCENP-B/HCP-4 and upstream of the outer kinetochore components HIM-10/Nuf2p, NDC-80/HEC1, CeBUB-1, HCP-1, and CeCLASP2/CLS-2; in expression studies in the one-cell embryo, KNL-1 localizes to kinetochores throughout mitosis.
Evidence for Inhibition 
ePCR Status: Unique_ePCR (III:8253230..8254026)
Transcript overlap: 629 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 608
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "C02F5.1" Links  Wormbase NCBI Phenobank 1
Cenix:307-c1 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance None reported
Phenotype  Sister Chromatid Separation abnormal (Cross-eyed)
External Movies 
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Cenix:307-c1 Experiment Details Ace View
Laboratory  TH
RNAi Reagent & Type  Cenix:307-c1 (PCR_reagent)
Remark  early embryonic: Sister Chromatid Separation (Cross-eyed)
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
Delivery Method  Injection
Date  2005-3-24
Gene Plot of C02F5.1 Browse using GBrowse
Gene Graph