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RNAi Experiment: Cenix:314-g1 Ace View of Cenix:314-g1
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  T04A8.14 (emb-5)
Description of "T04A8.14"  emb-5 encodes the C. elegans ortholog of the Spt6 family of RNA polymerase II transcription elongation factors; first identified in screens for temperature-sensitive embryonic lethal mutations, emb-5 is required for the correct timing of the second E (endoderm)-cell division in the early embryo and thus, for normal embryonic gastrulation; in addition, emb-5 activity is required postembryonically for proper gonad development; in yeast two-hybrid studies, EMB-5 interacts with the intracellular domains of LIN-12 and GLP-1, suggesting that EMB-5 functions as a positive downstream effector in Notch-like signaling pathways during C. elegans development; emb-5 mRNA is most abundant during embryonic stages, with lower levels apparent during the L1-L3 larval stages, and even lower levels observed during L4.
KOG Annotation  Transcription elongation factor SPT6
What's a KOG?
Evidence for Inhibition 
ePCR Status: Unique_ePCR (III:4717903..4719526)
Transcript overlap: 1480 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 1396
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "T04A8.14" Links  Wormbase NCBI Phenobank 1
Cenix:314-g1 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance None reported
Phenotype  WT_postembryonic_morphology
External Movies 
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Cenix:314-g1 Experiment Details Ace View
Laboratory  TH
RNAi Reagent & Type  Cenix:314-g1 (PCR_reagent)
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
Delivery Method  Injection
Date  2005-3-24
Gene Plot of T04A8.14 Browse using GBrowse
Gene Graph