RNAi Card Page

RNAi Experiment: Cenix:320-b9

Genes Inhibited

Gene Inhibited

Description of "K08E7.3"

let-99 encodes a protein with a DEP domain (Domain found in Dishevelled, Egl-10, and Pleckstrin); LET-99 has no obvious homologs in non-nematodes, but has a truncated paralog (LRG-1) in C. elegans; LET-99 is required for the mitotic spindle to be properly oriented with respect to the axis of cellular polarity, both during anterior migration and rotation of the nuclear-centrosome complex and during anaphase; let-99 mutants exhibit hyperactive nuclear movement and abnormal anaphase spindle pole behavior; LET-99 is mislocalized in par-2 and par-3 mutants; like other proteins containing DEP domains, LET-99 may act as part of a G protein signalling pathway (e.g., the one controlling spindle position).

Evidence for Inhibition

Sliding Window	Raw score: 172 Relative score: 0.151 (Best raw score= for Gene ) Specificity index: 0.131
Wormbase (BLAST)	RNAi secondary

External "K08E7.3" Links

Gene Inhibited

F55H2.4 (lrg-1)

Description of "F55H2.4"

lrg-1 encodes a truncated paralog of let-99, a protein with a DEP domain (Domain found in Dishevelled, Egl-10, and Pleckstrin); lrg-1(RNAi) animals have no obvious phenotype; since the nucleotide similarity of lrg-1 to let-99 extends beyond the lrg-1 CDS, and since lrg-1 has no obvious function in RNAi assays, this gene may be decaying towards pseudogene status.

Evidence for Inhibition

Sliding Window	Raw score: 1137 Relative score: 1 (This gene has the best score for this RNAi reagent) Specificity index: 0.869
Wormbase (BLAST)	RNAi primary

External "F55H2.4" Links

Cenix:320-b9 Phenotypes