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RNAi Experiment: Cenix:322-d11 Ace View of Cenix:322-d11
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  F54C8.2
Description of "F54C8.2"  Associated GO Term : DNA binding
KOG Annotation  Histones H3 and H4
What's a KOG?
Evidence for Inhibition 
ePCR Status: Best_ePCR (III:9433793..9435114)
Transcript overlap: 786 nt		 Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 702
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.807
Wormbase (BLAST) RNAi primary
External "F54C8.2" Links  Wormbase NCBI Phenobank 1
Gene Inhibited  F54C8.3 (emb-30)
Description of "F54C8.3"  emb-30 encodes an anaphase-promoting complex/cyclosome (APC/C) component orthologous to mammalian APC-4 and Schizosaccharomyces pombe Lid1; EMB-30 is required for the metaphase-to-anaphase transition during meiosis and mitosis, for establishing anterior-posterior polarity in the early embryo, and for proper localization of germline granules and the maternally provided PAR-2 and PAR-3 proteins.
Evidence for Inhibition 
ePCR Status: Bottom50_percentile_ePCR (III:9433793..9435114)
Transcript overlap: 136 nt		 Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 94
Relative score: 0.134 (Best raw score= for Gene )
Specificity index: 0.108
External "F54C8.3" Links  Wormbase NCBI Phenobank 1
Gene Inhibited  F58A4.3 (hcp-3)
Description of "F58A4.3"  The hcp-3 gene encodes a centromere protein (CENP)-A homolog required for kinetochore function; inactivation of hcp-3 in one-cell embryos by RNAi causes a complete loss of kinetochores, with total failure of chromosomes to segregate properly during mitosis, to recruit components to the kinetochore other than HCP-3, or to assemble a stable mitotic spindle; in addition, HCP-4 fails to localize properly to the kinetochore in hcp-3(RNAi) embryos.
KOG Annotation  Histones H3 and H4
What's a KOG?
Evidence for Inhibition 
Sliding Window Raw score: 74
Relative score: 0.105 (Best raw score= for Gene )
Specificity index: 0.085
Wormbase (BLAST) RNAi secondary
External "F58A4.3" Links  Wormbase NCBI Phenobank 1
Cenix:322-d11 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance None reported
Phenotype  Chromosome Segregation (karyomeres) abnormal
External Movies 
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Cenix:322-d11 Experiment Details Ace View
Laboratory  TH
RNAi Reagent & Type  Cenix:322-d11 (PCR_reagent)
Remark  early embryonic: Chromosome Segregation (karyomeres)
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
Delivery Method  Injection
Date  2005-3-24
Gene Plot of F54C8.3 Browse using GBrowse
Gene Graph
Gene Plot of F58A4.3 Browse using GBrowse
Gene Graph
Gene Plot of F54C8.2 Browse using GBrowse
Gene Graph