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RNAi Experiment: Cenix:335-f3 Ace View of Cenix:335-f3
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  F58A4.3 (hcp-3)
Description of "F58A4.3"  The hcp-3 gene encodes a centromere protein (CENP)-A homolog required for kinetochore function; inactivation of hcp-3 in one-cell embryos by RNAi causes a complete loss of kinetochores, with total failure of chromosomes to segregate properly during mitosis, to recruit components to the kinetochore other than HCP-3, or to assemble a stable mitotic spindle; in addition, HCP-4 fails to localize properly to the kinetochore in hcp-3(RNAi) embryos.
KOG Annotation  Histones H3 and H4
What's a KOG?
Evidence for Inhibition 
ePCR Status: Best_ePCR (III:9614933..9616513)
Transcript overlap: 1010 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 947
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "F58A4.3" Links  Wormbase NCBI Phenobank 1 2
Gene Inhibited  F58A4.6
Evidence for Inhibition 
ePCR Status: Bottom50_percentile_ePCR (III:9614933..9616513)
Transcript overlap: 92 nt
Explain types of evidence for RNAi inhibition
Wormbase (BLAST) RNAi primary
External "F58A4.6" Links  Wormbase NCBI Phenobank 1 2
Cenix:335-f3 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance None reported
Phenotype  Sister Chromatid Separation abnormal (Cross-eyed)
External Movies 
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Cenix:335-f3 Experiment Details Ace View
Laboratory  TH
RNAi Reagent & Type  Cenix:335-f3 (PCR_reagent)
Remark  early embryonic: Sister Chromatid Separation (Cross-eyed)
Reference  Sonnichsen B et al. (2005) Nature "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis ...."
Delivery Method  Injection
Date  2005-3-24
Gene Plot of F58A4.3 Browse using GBrowse
Gene Graph
Gene Plot of F58A4.6 Browse using GBrowse
Gene Graph