RNAi Card Page

RNAi Experiment: Cenix:46-e4

Genes Inhibited

Gene Inhibited

Description of "Y116A8C.32"

Y116A8C.32 encodes an ortholog of splicing factor SF1, which enables 3' splice site recognition by binding U2AF65 and the intron branch site during splicing complex formation; Y116A8C.32 shares several domains with mammalian SF1 proteins (a U2AF65 binding domain, a hnRNP K homology domain, two RNA-binding zinc knuckles, and a proline-rich C-terminal domain), while also sharing a hydrophilic N-terminal domain (enriched for serine, arginine, lysine, and aspartate) with Drosophila but not mammalian SF1; Y116A8C.32's N-terminal domain resembles RS domains in other splicing proteins; Y116A8C.32 is required for embryonic viability, normally rapid growth, and proper body morphology.

KOG Annotation

Splicing factor 1/branch point binding protein (RRM superfamily)

Evidence for Inhibition

ePCR	Status: Unique_ePCR (IV:17104688..17106120) Transcript overlap: 636 nt
Sliding Window	Raw score: 594 Relative score: 1 (This gene has the best score for this RNAi reagent) Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST)	RNAi primary