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RNAi Experiment: JA:F32H2.3 Ace View of JA:F32H2.3
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  F32H2.3 (spd-2)
Description of "F32H2.3"  spd-2 encodes a protein with three predicted coiled-coil domains; SPD-2 activity is essential for centrosome maturation and duplication, and hence for proper mitotic spindle assembly; in regulating the early steps of centrosome maturation, SPD-2 acts together with the coiled-coil protein SPD-5, the Aurora-A kinase AIR-1, and the cytoplasmic dynein DHC-1; in regulating centrosome duplication, SPD-2 likely acts together with the ZYG-1 kinase; in both processes, SPD-2 may function to recruit centrosomal components such as ZYG-9 and microtubule-nucleating gamma-tubulin complexes to the developing centrosome; within centrosomes, SPD-2 localizes to both centrioles and the pericentriolar material; efficient centrosomal localization depends upon AIR-1 and DHC-1, while localization specifically to the pericentriolar material depends upon SPD-5.
Evidence for Inhibition 
ePCR Status: Unique_ePCR (I:8969088..8970166)
Transcript overlap: 900 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 816
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "F32H2.3" Links  Wormbase NCBI
JA:F32H2.3 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance 100 %
Phenotype  Spd 1-12 cell arrest
Movies/Stacks/Images  /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif Help
JA:F32H2.3 Experiment Details Ace View
Laboratory  JA
RNAi Reagent & Type  sjj_F32H2.3 (PCR_reagent)
Remark  small abnormal spindle
Reference  Fraser AG et al. (2000) Nature "Functional genomic analysis of C. elegans chromosome I by systematic RNA ...."
Date  2000-11-16
Gene Plot of F32H2.3 Browse using GBrowse
Gene Graph