Link to F53G12.3RNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentLink to F56C11.1geneMap Link to F56C11.1RNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentLink to F53G12.3geneMap
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RNAi Experiment: JA:F53G12.3 Ace View of JA:F53G12.3
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  F53G12.3
Description of "F53G12.3"  F53G12.3 encodes a large partial homolog of dual oxidase ('Ce-Duox2'), with an N-terminal peroxidase domain, two central calmodulin-binding EF hands, and a C-terminal superoxide-generating NADPH-oxidase domain; F53G12.3 may be required for dityrosine cross-linking of collagen, and thus for cuticular integrity; F53G12.3 may use cytosolic NADPH to generate reactive oxygen, which then may drive the peroxidase ectodomain to cross-link free tyrosine in collagen; F53G12.3 has no visible expression pattern detectable by antibodies, implying very low or rare expression.
KOG Annotation  Ferric reductase, NADH/NADPH oxidase and related proteins
What's a KOG?
Evidence for Inhibition 
ePCR Status: Unique_ePCR (I:138837..140015)
Transcript overlap: 954 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 830
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.638
Wormbase (BLAST) RNAi primary
External "F53G12.3" Links  Wormbase NCBI
Gene Inhibited  F56C11.1 (bli-3)
Description of "F56C11.1"  bli-3 encodes a large homolog of dual oxidase ('Ce-Duox1'), with an N-terminal peroxidase domain, two central calmodulin-binding EF hands, and a C-terminal superoxide-generating NADPH-oxidase domain; BLI-3 is required for dityrosine cross-linking of collagen, and thus for cuticular integrity; BLI-3 is thought to use cytosolic NADPH to generate reactive oxygen, which then drives the peroxidase ectodomain to cross-link free tyrosine in collagen; BLI-3 is expressed exclusively in hypodermal cells at low levels, with peaks of expression corresponding to collagen/cuticle biosynthesis.
KOG Annotation  Ferric reductase, NADH/NADPH oxidase and related proteins
What's a KOG?
Evidence for Inhibition 
Sliding Window Raw score: 471
Relative score: 0.567 (Best raw score= for Gene )
Specificity index: 0.362
Wormbase (BLAST) RNAi primary
External "F56C11.1" Links  Wormbase NCBI
JA:F53G12.3 Phenotypes Help with Phenotypes
Larval  Lvl
Mlt
Phenotype  Bli
JA:F53G12.3 Experiment Details Ace View
Laboratory  JA
RNAi Reagent & Type  sjj_F53G12.3 (PCR_reagent)
Reference  Fraser AG et al. (2000) Nature "Functional genomic analysis of C. elegans chromosome I by systematic RNA ...."
Date  2000-11-16
Gene Plot of F56C11.1 Browse using GBrowse
Gene Graph
Gene Plot of F53G12.3 Browse using GBrowse
Gene Graph