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RNAi Experiment: JA:F54C1.3 Ace View of JA:F54C1.3
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  F54C1.3 (mes-3)
Description of "F54C1.3"  mes-3 encodes a novel protein with no discernible motifs; however, as a member of a Polycomb-like chromatin repressive complex with MES-2 and MES-6, MES-3 is required maternally for normal germline development and during larval development, for anteroposterior patterning; during germline development, the MES-2/MES-3/MES-6 complex is believed to be essential for maintaining repression of the X chromosome, and in transgenic animals, the complex is necessary for germline repression of repetitive transgenes; in axial patterning, the MES-2/MES-3/MES-6 complex is required in somatic tissues for maintaining homeotic gene repression, acting upstream of the Hox genes lin-39, mab-5, and egl-5, as well as the egl-5 target gene lin-32; MES-3 expression is detected in the nuclei of all cells during early embryogenesis, in some somatic cells and the germline precursors Z2 and Z3 during late embryogenesis and the L1 larval stage; in adults, MES-3 is expressed at highest levels in oocytes and germline nuclei in the proximal meiotic region with expression in the pachytene region negatively regulated by the RNA-binding protein GLD-1; MES-3 appears to be localized primarily to the nucleoplasm with some chromosomal association detectable during metaphase and early anaphase; in >24-cell-stage embryos, normal MES-3 levels and localization are dependent upon wild-type activity of MES-2 and MES-6
Evidence for Inhibition 
ePCR Status: Unique_ePCR (I:4999572..5001095)
Transcript overlap: 702 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 597
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "F54C1.3" Links  Wormbase NCBI
JA:F54C1.3 Phenotypes Help with Phenotypes
Adult  Stp
JA:F54C1.3 Experiment Details Ace View
Laboratory  JA
RNAi Reagent & Type  sjj_F54C1.3 (PCR_reagent)
Reference  Fraser AG et al. (2000) Nature "Functional genomic analysis of C. elegans chromosome I by systematic RNA ...."
Date  2000-11-16
Gene Plot of F54C1.3 Browse using GBrowse
Gene Graph