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RNAi Experiment: JA:M7.1 Ace View of JA:M7.1
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  M7.1 (let-70)
Description of "M7.1"  let-70 encodes a class I E2 ubiquitin conjugating enzyme; let-70 was identified in screens for essential genes and phenotypic analyses indicate that loss of let-70 activity results in embryonic and larval lethality with defects in sarcomere assembly; purified LET-70 can stimulate the self-ubiquitylation activities of CHN-1 and UFD-2, E4 ubiquitin conjugation factors and, in addition, can stimulate the CHN-1- and UFD-2-dependent multiubiquitylation of UNC-45, a myosin-directed chaperone; in this manner, LET-70 may regulate UNC-45 degradation and myosin assembly; RNA blot analyses and a let-70-lacZ reporter fusion reveal that let-70 is abundantly expressed at all stages of development, including the dauer larval stage; strong staining is observed in most somatic tissues until adult stages, when expression generally becomes restricted to the nervous system.
KOG Annotation  Ubiquitin-protein ligase
What's a KOG?
Evidence for Inhibition 
ePCR Status: Unique_ePCR (IV:11081430..11082308)
Transcript overlap: 610 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 505
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.847
Wormbase (BLAST) RNAi primary
External "M7.1" Links  Wormbase NCBI
Gene Inhibited  F52C6.12
Description of "F52C6.12"  Associated GO Term : ubiquitin-like activating enzyme activity
Evidence for Inhibition 
Sliding Window Raw score: 91
Relative score: 0.18 (Best raw score= for Gene )
Specificity index: 0.153
Wormbase (BLAST) RNAi primary
External "F52C6.12" Links  Wormbase NCBI
JA:M7.1 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance 100 %
Adult  Pvl
Phenotype  Adl
JA:M7.1 Experiment Details Ace View
Laboratory  JA
RNAi Reagent & Type  sjj_M7.1 (PCR_reagent)
Reference  Kamath RS et al. (2003) Nature "Systemic functional analysis of the Caenorhabditis elegans genome using ...."
Date  2003-1-10
Gene Plot of F52C6.12 Browse using GBrowse
Gene Graph
Gene Plot of M7.1 Browse using GBrowse
Gene Graph