RNAi Card Page

RNAi Experiment: JA:Y55D5A_391.b

Genes Inhibited

Gene Inhibited

Description of "Y55D5A.5"

daf-2 encodes a predicted receptor tyrosine kinase that is the C. elegans insulin/IGF receptor ortholog; DAF-2 activity is required for a number of processes in C. elegans, including embryonic and larval development, formation of the developmentally arrested dauer larval stage, adult longevity, reproduction, fat storage, and response to exogenous stressors such as high temperature or bacterial infection; DAF-2 signals through a conserved PI 3-kinase pathway to negatively regulate the activity of DAF-16, a Forkhead-related transcription factor, by inducing its phosphorylation and nuclear exclusion; amongst the 38 predicted insulin-like molecules in C. elegans, genetic and microarray analyses suggest that at least DAF-28, INS-1, and INS-7 are likely DAF-2 ligands; genetic mosaic and tissue-specific promoter studies indicate that daf-2 can function cell nonautonomously and within multiple cell types to influence dauer formation and adult lifespan, likely by regulating the production of secondary signals that coordinate growth and longevity throughout the animal.

KOG Annotation

Insulin/growth factor receptor (contains protein kinase domain)

Evidence for Inhibition

ePCR	Status: Unique_ePCR (III:3012401..3014686) Transcript overlap: 1277 nt
Sliding Window	Raw score: 1214 Relative score: 1 (This gene has the best score for this RNAi reagent) Specificity index: 0.829
Wormbase (BLAST)	RNAi primary