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RNAi Experiment: KK:SP_12E1 Ace View of KK:SP_12E1
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  T04A8.14 (emb-5)
Description of "T04A8.14"  emb-5 encodes the C. elegans ortholog of the Spt6 family of RNA polymerase II transcription elongation factors; first identified in screens for temperature-sensitive embryonic lethal mutations, emb-5 is required for the correct timing of the second E (endoderm)-cell division in the early embryo and thus, for normal embryonic gastrulation; in addition, emb-5 activity is required postembryonically for proper gonad development; in yeast two-hybrid studies, EMB-5 interacts with the intracellular domains of LIN-12 and GLP-1, suggesting that EMB-5 functions as a positive downstream effector in Notch-like signaling pathways during C. elegans development; emb-5 mRNA is most abundant during embryonic stages, with lower levels apparent during the L1-L3 larval stages, and even lower levels observed during L4.
KOG Annotation  Transcription elongation factor SPT6
What's a KOG?
Evidence for Inhibition 
Sliding Window Raw score: 835
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
External "T04A8.14" Links  Wormbase NCBI
KK:SP_12E1 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance 80 - 100 %
Phenotype  P0 spindle positioning problems (posterior)
Polar bodies abnormal
Pseudocleavage problems
Movies/Stacks/Images  /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif Help
KK:SP_12E1 Experiment Details Ace View
Laboratory  KK
RNAi Reagent & Type  SP12E1 (cDNA_reagent)
Reference  Piano F et al. (2000) Current Biology "RNAi analysis of genes expressed in the ovary of Caenorhabditis ...."
Delivery Method  Injection
Date  2000-12-08
Gene Plot of T04A8.14 Browse using GBrowse
Gene Graph