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RNAi Experiment: KK:SP_6F12 Ace View of KK:SP_6F12
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  F35B12.5 (sas-5)
Description of "F35B12.5"  sas-5 encodes a coiled-coil protein whose activity is required, paternally, maternally, and in a dose-dependent manner, for daughter centriole formation; SAS-5 localizes to both the cytoplasm and to centrioles, and photobleaching experiments reveal rapid SAS-5 cycling between these two cellular components throughout the cell cycle; SAS-5 localization to centrioles depends upon the ZYG-1 kinase and the SAS-6 coiled-coil protein, two proteins also required for centriole duplication; in turn, SAS-5 activity is required for SAS-6 centriolar localization, as SAS-6 fails to localize properly in sas-5 mutant embryos.
Evidence for Inhibition 
Sliding Window Raw score: 1616
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
External "F35B12.5" Links  Wormbase NCBI
KK:SP_6F12 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance 80 - 100 %
Phenotype  Abnormal blebbing
Cytokinesis problems
Multiple nuclei
Movies/Stacks/Images  /static/images/movie.gif /static/images/movie.gif Help
KK:SP_6F12 Experiment Details Ace View
Laboratory  KK
RNAi Reagent & Type  SP6F12 (cDNA_reagent)
Reference  Piano F et al. (2000) Current Biology "RNAi analysis of genes expressed in the ovary of Caenorhabditis ...."
Delivery Method  Injection
Date  2000-12-08
Gene Plot of F35B12.5 Browse using GBrowse
Gene Graph