RNAi Experiment: MV_SV:mv_AAB25489 |
Ace View of MV_SV:mv_AAB25489
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Genes Inhibited |
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Gene Inhibited |
M7.1 (let-70) |
Description of "M7.1" |
let-70 encodes a class I E2 ubiquitin conjugating enzyme; let-70 was identified in screens for essential genes and phenotypic analyses indicate that loss of let-70 activity results in embryonic and larval lethality with defects in sarcomere assembly; purified LET-70 can stimulate the self-ubiquitylation activities of CHN-1 and UFD-2, E4 ubiquitin conjugation factors and, in addition, can stimulate the CHN-1- and UFD-2-dependent multiubiquitylation of UNC-45, a myosin-directed chaperone; in this manner, LET-70 may regulate UNC-45 degradation and myosin assembly; RNA blot analyses and a let-70-lacZ reporter fusion reveal that let-70 is abundantly expressed at all stages of development, including the dauer larval stage; strong staining is observed in most somatic tissues until adult stages, when expression generally becomes restricted to the nervous system. |
KOG Annotation  |
Ubiquitin-protein ligase
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Evidence for Inhibition |
Sliding Window |
Raw score: 336
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.826 |
Wormbase (BLAST) |
RNAi primary |
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External "M7.1" Links |
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Gene Inhibited |
F52C6.12 |
Description of "F52C6.12" |
Associated GO Term : ubiquitin-like activating enzyme activity |
Evidence for Inhibition |
Sliding Window |
Raw score: 71
Relative score: 0.211 (Best raw score= for Gene )
Specificity index: 0.174 |
Wormbase (BLAST) |
RNAi primary |
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External "F52C6.12" Links |
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MV_SV:mv_AAB25489 Phenotypes |
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Embryonic |
Emb Penetrance None reported |
Larval |
Lva
phenotype observed among F1 progeny of treated animals
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Adult |
Sck Unc
penetrance is less than 90%
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Phenotype |
Unclassified
reduced brood size
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MV_SV:mv_AAB25489 Experiment Details |
Ace View |
Laboratory |
MV |
RNAi Reagent & Type |
mv_AAB25489 (ORFeome_reagent) |
Remark |
bacterial-feeding RNAi began at the L1 larval stage |
Reference |
Rual JF et al. (2004) Genome Res "Toward Improving Caenorhabditis elegans Phenome Mapping With an ORFeome-Based ...." |
Delivery Method |
Bacterial_feeding |
Date |
2004-9-01 |
Gene Plot of F52C6.12 |
Browse using GBrowse
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Gene Plot of M7.1 |
Browse using GBrowse
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