RNAi Card Page

RNAi Experiment: MV_SV:mv_C09G5.4

Genes Inhibited

Gene Inhibited

Description of "ZK1193.1"

col-19 encodes a member of the collagen superfamily containing collagen triple helix repeats (20 copies) that is required for normal structure of the alae; expressed during the L2-to-dauer and L4-to-adult molts with strongest expression in adult animals.

KOG Annotation

Collagens (type IV and type XIII), and related proteins

Evidence for Inhibition

Sliding Window

Raw score: 379
Relative score: 0.418 (Best raw score= for Gene )
Specificity index: 0.137

External "ZK1193.1" Links

Gene Inhibited

W01B6.7 (col-2)

Description of "W01B6.7"

col-2 encodes a member of the collagen superfamily containing collagen triple helix repeats (20 copies); expression peaks during the molt that separates the L2 larval and dauer stages as the dauer cuticle is being formed, and mRNA is expressed at low levels in post-dauer L4 larvae and in adult animals.

KOG Annotation

Collagens (type IV and type XIII), and related proteins

Evidence for Inhibition

Sliding Window

Raw score: 66
Relative score: 0.073 (Best raw score= for Gene )
Specificity index: 0.024

External "W01B6.7" Links

Gene Inhibited

T11B7.3 (col-118)

Description of "T11B7.3"

Associated GO Term : structural constituent of cuticle

KOG Annotation

Collagens (type IV and type XIII), and related proteins

Evidence for Inhibition

Sliding Window

Raw score: 101
Relative score: 0.111 (Best raw score= for Gene )
Specificity index: 0.037

External "T11B7.3" Links

Gene Inhibited

Y2H9A.3 (col-162)

Description of "Y2H9A.3"

Associated GO Term : structural constituent of cuticle

KOG Annotation

Collagens (type IV and type XIII), and related proteins

Evidence for Inhibition

Sliding Window

Raw score: 61
Relative score: 0.067 (Best raw score= for Gene )
Specificity index: 0.022

External "Y2H9A.3" Links

Gene Inhibited

F57B7.3 (col-156)

Description of "F57B7.3"

Associated GO Term : structural constituent of cuticle

KOG Annotation

Collagens (type IV and type XIII), and related proteins

Evidence for Inhibition

Sliding Window

Raw score: 302
Specificity index: 0.109

External "F57B7.3" Links

Gene Inhibited

T21B4.2 (col-85)

Description of "T21B4.2"

Associated GO Term : structural constituent of cuticle

KOG Annotation

Collagens (type IV and type XIII), and related proteins

Evidence for Inhibition

Sliding Window

Raw score: 319
Relative score: 0.352 (Best raw score= for Gene )
Specificity index: 0.115

External "T21B4.2" Links

Gene Inhibited

F38A3.2 (ram-2)

Description of "F38A3.2"

ram-2 encodes a cuticle collagen that interacts with unc-6 to affect ray cell migration, and interacts with unc-5 and unc-6 to affect embryonic viability; also affects ray morphology in males such that the structural cells and the hypodermis of the rays are swollen in mutants.

KOG Annotation

Collagens (type IV and type XIII), and related proteins

Evidence for Inhibition

Sliding Window

Raw score: 302
Relative score: 0.333 (Best raw score= for Gene )
Specificity index: 0.109

External "F38A3.2" Links

Gene Inhibited

H27M09.4 (dpy-14)

Description of "H27M09.4"

dpy-14 encodes a type III (alpha 1) collagen that is required for embryonic, larval, and vulval development, proper amphid morphology, and regulation of body shape and size; a dpy-14 promoter-GFP fusion construct is reportedly expressed in embryonic neurons.

KOG Annotation

Collagens (type IV and type XIII), and related proteins

Evidence for Inhibition

Sliding Window

Raw score: 328
Relative score: 0.362 (Best raw score= for Gene )
Specificity index: 0.119

External "H27M09.4" Links

Gene Inhibited

C09G5.4 (col-39)

Description of "C09G5.4"

col-39 encodes a collagen that is individually dispensable for viability and gross morphology in mass RNAi screens; the amino- and carboxyl-terminal cysteine-rich regions of COL-39 are most closely related to those of COL-8, COL-19, and COL-35.

KOG Annotation

Collagens (type IV and type XIII), and related proteins

Evidence for Inhibition

Sliding Window	Raw score: 906 Relative score: 1 (This gene has the best score for this RNAi reagent) Specificity index: 0.328
Wormbase (BLAST)	RNAi primary

External "C09G5.4" Links

MV_SV:mv_C09G5.4 Phenotypes

Phenotype

WTRNAi did not cause viable post-embryonic phenotype

MV_SV:mv_C09G5.4 Experiment Details

Ace View

Laboratory

RNAi Reagent & Type

mv_C09G5.4 (ORFeome_reagent)

Remark

bacterial-feeding RNAi began at the L1 larval stage

Reference