Link to K08E7.3Link to K08E7.4RNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentLink to F55H2.4Link to Y53F4A.2geneMap RNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentLink to K08E7.3geneMap
Go to RNAiDB Home Adult Wild-type C. elegans Movie Wild-type C. elegans Movie Wild-type C. elegans Movie Wild-type C. elegans Movie
 
RNAi Experiment: MV_SV:mv_K08E7.3 Ace View of MV_SV:mv_K08E7.3
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  K08E7.3 (let-99)
Description of "K08E7.3"  let-99 encodes a protein with a DEP domain (Domain found in Dishevelled, Egl-10, and Pleckstrin); LET-99 has no obvious homologs in non-nematodes, but has a truncated paralog (LRG-1) in C. elegans; LET-99 is required for the mitotic spindle to be properly oriented with respect to the axis of cellular polarity, both during anterior migration and rotation of the nuclear-centrosome complex and during anaphase; let-99 mutants exhibit hyperactive nuclear movement and abnormal anaphase spindle pole behavior; LET-99 is mislocalized in par-2 and par-3 mutants; like other proteins containing DEP domains, LET-99 may act as part of a G protein signalling pathway (e.g., the one controlling spindle position).
Evidence for Inhibition 
Sliding Window Raw score: 1989
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.868
Wormbase (BLAST) RNAi primary
External "K08E7.3" Links  Wormbase NCBI
Gene Inhibited  F55H2.4 (lrg-1)
Description of "F55H2.4"  lrg-1 encodes a truncated paralog of let-99, a protein with a DEP domain (Domain found in Dishevelled, Egl-10, and Pleckstrin); lrg-1(RNAi) animals have no obvious phenotype; since the nucleotide similarity of lrg-1 to let-99 extends beyond the lrg-1 CDS, and since lrg-1 has no obvious function in RNAi assays, this gene may be decaying towards pseudogene status.
Evidence for Inhibition 
Sliding Window Raw score: 303
Relative score: 0.152 (Best raw score= for Gene )
Specificity index: 0.132
Wormbase (BLAST) RNAi primary
External "F55H2.4" Links  Wormbase NCBI
MV_SV:mv_K08E7.3 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance None reported
MV_SV:mv_K08E7.3 Experiment Details Ace View
Laboratory  MV
RNAi Reagent & Type  mv_K08E7.3 (ORFeome_reagent)
Remark  bacterial-feeding RNAi began at the L1 larval stage
Reference  Rual JF et al. (2004) Genome Res "Toward Improving Caenorhabditis elegans Phenome Mapping With an ORFeome-Based ...."
Delivery Method  Bacterial_feeding
Date  2004-9-01
Gene Plot of K08E7.3 Browse using GBrowse
Gene Graph
Gene Plot of F55H2.4 Browse using GBrowse
Gene Graph