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RNAi Experiment: MV_SV:mv_ZC404.8 Ace View of MV_SV:mv_ZC404.8
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  ZC404.8 (spn-4)
Description of "ZC404.8"  spn-4 encodes a protein containing an RNP-type RNA-binding domain; SPN-4 is required for rotation, but not polarization, of the mitotic spindle in the P1 blastomere of the two-cell stage embryo; SPN-4 is also required for mesectoderm and mesendoderm formation in conjunction with proper localization of maternal cell-fate determinants such as SKN-1; in addition, SPN-4 binds the glp-1 3' UTR and is necessary for glp-1 mRNA translation in the anterior blastomeres of the early embryo; conversely, SPN-4 binds and suppresses translation of pal-1 and skn-1 mRNAs in anterior blastomeres; in vitro, SPN-4 interacts with POS-1, a CCCH-type zinc finger protein that negatively regulates glp-1 mRNA translation in posterior blastomeres; SPN-4 is a P granule component, but is also detected in oocytes, the AB and P1 blastomeres at the two-cell stage, and in all four blastomeres at the four-cell stage, with higher levels in EMS and P2.
KOG Annotation  Ataxin 2-binding protein (RRM superfamily)
What's a KOG?
Evidence for Inhibition 
Sliding Window Raw score: 948
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "ZC404.8" Links  Wormbase NCBI
MV_SV:mv_ZC404.8 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance None reported
MV_SV:mv_ZC404.8 Experiment Details Ace View
Laboratory  MV
RNAi Reagent & Type  mv_ZC404.8 (ORFeome_reagent)
Remark  bacterial-feeding RNAi began at the L1 larval stage
Reference  Rual JF et al. (2004) Genome Res "Toward Improving Caenorhabditis elegans Phenome Mapping With an ORFeome-Based ...."
Delivery Method  Bacterial_feeding
Date  2004-9-01
Gene Plot of ZC404.8 Browse using GBrowse
Gene Graph