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RNAi Experiment: PF:GL1_1A12 Ace View of PF:GL1_1A12
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  F22B3.4
Description of "F22B3.4"  Associated GO Term : metabolism
KOG Annotation  Glucosamine 6-phosphate synthetases, contain amidotransferase and phosphosugar isomerase domains
What's a KOG?
Evidence for Inhibition 
ePCR Status: Unique_ePCR (IV:11411918..11412845)
Transcript overlap: 827 nt		 Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 764
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "F22B3.4" Links  Wormbase NCBI
PF:GL1_1A12 Phenotypes Run Phenoblast! Run Phenoblast Help with Phenotypes
Embryonic  Emb Penetrance 80 - 100 %
Adult  Ste
No developing embryos seen 48 hours after dsRNA injection
Reduced fecundity of injected worm
Phenotype  Abnormal one-cell shape
Catastrophic one-cell arrest
Cytokinesis defect
Multiple nuclei in 1, 2, or 4-cell embryos
Osmotically/ pressure sensitive
Polar body reabsorbed
Movies/Stacks/Images  /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif Help
PF:GL1_1A12 Experiment Details Ace View
Laboratory  KK
RNAi Reagent & Type  sjj_F22B3.4 (PCR_reagent)
Remark  PCR product contains flanking T7 promoter sequences
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
Delivery Method  Injection
Date  2002-11-19
Gene Plot of F22B3.4 Browse using GBrowse
Gene Graph