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RNAi Experiment: PF:GL1_1B2 Ace View of PF:GL1_1B2
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  T25G3.2 (chs-1)
Description of "T25G3.2"  chi-1 encodes a putative chitin synthase, most closely related to chitin synthases characterized in filarial nematodes.
Evidence for Inhibition 
ePCR Status: Unique_ePCR (I:7557913..7558939)
Transcript overlap: 883 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 801
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "T25G3.2" Links  Wormbase NCBI
PF:GL1_1B2 Phenotypes Run Phenoblast! Run Phenoblast Help with Phenotypes
Embryonic  Emb Penetrance 100 %
Adult  Ste
No developing embryos seen 48 hours after dsRNA injection
Reduced fecundity of injected worm
PF:GL1_1B2 Experiment Details Ace View
Laboratory  KK
RNAi Reagent & Type  sjj_T25G3.2 (PCR_reagent)
Remark  PCR product contains flanking T7 promoter sequences
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
Delivery Method  Injection
Date  2002-11-19
Gene Plot of T25G3.2 Browse using GBrowse
Gene Graph