Ace View of PF:GL1_1C4 | |||
Gene Inhibited | W03D2.4 (pcn-1) | ||
Description of "W03D2.4" | pcn-1 encodes the C. elegans ortholog of proliferating cell nuclear antigen (PCNA), an acidic protein that is an essential component of the DNA replication and repair machinery and serves as a processivity factor for DNA polymerase delta; PCN-1 is required for early embryogenesis and for proper germ-line development, and directly interacts with CKI-2, a cyclin-dependent kinase inhibitor homolog that may be required for cell cycle progression during embryogenesis, and with ATL-1/PI3K, KIN-20, MRT-2/RAD1, and RFC-1, proteins that all play a role in the DNA damage checkpoint pathway; PCN-1 is highly expressed in ovaries and developing embryos. | ||
KOG Annotation  | DNA polymerase delta processivity factor (proliferating cell nuclear antigen) |
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Evidence for Inhibition | |||
External "W03D2.4" Links | |||
Embryonic | Emb Penetrance 100 % | ||
Adult | Ste Reduced fecundity of injected worm |
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Phenotype | Aberrant nuclear reassembly Cytokinesis defect Ectopic cleavage furrows Excessive blebbing Multiple cavities Multiple nuclei in 1, 2, or 4-cell embryos Polar body reabsorbed Slow cell cycle |
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Movies/Stacks/Images | |||
Laboratory | KK | ||
RNAi Reagent & Type | sjj_W03D2.4 (PCR_reagent) | ||
Remark | PCR product contains flanking T7 promoter sequences | ||
Reference | Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans" | ||
Delivery Method | Injection | ||
Date | 2002-11-19 | ||
Gene Plot of W03D2.4 | |||