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RNAi Experiment: PF:GL1_1C4 Ace View of PF:GL1_1C4
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  W03D2.4 (pcn-1)
Description of "W03D2.4"  pcn-1 encodes the C. elegans ortholog of proliferating cell nuclear antigen (PCNA), an acidic protein that is an essential component of the DNA replication and repair machinery and serves as a processivity factor for DNA polymerase delta; PCN-1 is required for early embryogenesis and for proper germ-line development, and directly interacts with CKI-2, a cyclin-dependent kinase inhibitor homolog that may be required for cell cycle progression during embryogenesis, and with ATL-1/PI3K, KIN-20, MRT-2/RAD1, and RFC-1, proteins that all play a role in the DNA damage checkpoint pathway; PCN-1 is highly expressed in ovaries and developing embryos.
KOG Annotation  DNA polymerase delta processivity factor (proliferating cell nuclear antigen)
What's a KOG?
Evidence for Inhibition 
ePCR Status: Unique_ePCR (IV:4048059..4049606)
Transcript overlap: 862 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 799
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "W03D2.4" Links  Wormbase NCBI
PF:GL1_1C4 Phenotypes Run Phenoblast! Run Phenoblast Help with Phenotypes
Embryonic  Emb Penetrance 100 %
Adult  Ste
Reduced fecundity of injected worm
Phenotype  Aberrant nuclear reassembly
Cytokinesis defect
Ectopic cleavage furrows
Excessive blebbing
Multiple cavities
Multiple nuclei in 1, 2, or 4-cell embryos
Polar body reabsorbed
Slow cell cycle
Movies/Stacks/Images  /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif Help
PF:GL1_1C4 Experiment Details Ace View
Laboratory  KK
RNAi Reagent & Type  sjj_W03D2.4 (PCR_reagent)
Remark  PCR product contains flanking T7 promoter sequences
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
Delivery Method  Injection
Date  2002-11-19
Gene Plot of W03D2.4 Browse using GBrowse
Gene Graph