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RNAi Experiment: PF:GL1_3A1 Ace View of PF:GL1_3A1
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  F49E11.1 (mbk-2)
Description of "F49E11.1"  mbk-2 encodes one of two C. elegans members of the DYRK (dual-specificity Yak1-related kinase) family of proteins that includes S. cerevisiae Yak1 and the Drosophila minibrain and DYRK2 kinases; MBK-2 activity is required maternally for the egg-to-embryo transition that occurs during the earliest stages of embryonic development; specifically, MBK-2 is required for: 1) posterior localization of the germ plasm components PIE-1, POS-1, and PGL-1, and 2) postfertilization degradation of a subset of maternal proteins including the MEI-1 and MEI-2 meiosis-specific katanin subunits, the OMA-1 oocyte maturation factor, and residual PIE-1 that remains anteriorly localized after its normal posterior segregation; genetic analyses suggest that, in regulating the segregation and degradation of maternal proteins, mbk-2 lies downstream of the initial embryonic polarity cues established by the PAR/MEX proteins; MBK-2, whose activity appears to depend upon progression through the meiotic divisions, is expressed uniformly in the cortex of oocytes and newly fertilized zygotes; in later zygotes, just prior to the second meiotic division, MBK-2 becomes localized to discrete cortical foci, and by the first mitosis it is found predominantly on centrosomes and chromosomes; MBK-2 is also associated with P granules in the germline blastomeres P2, P3, and P4.
KOG Annotation  Dual-specificity tyrosine-phosphorylation regulated kinase
What's a KOG?
Evidence for Inhibition 
ePCR Status: Unique_ePCR (IV:13033003..13034018)
Transcript overlap: 963 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 921
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "F49E11.1" Links  Wormbase NCBI
PF:GL1_3A1 Phenotypes Run Phenoblast! Run Phenoblast Help with Phenotypes
Embryonic  Emb Penetrance 80 - 100 %
Adult  Dpy Observed in <10% of progeny.
Rup Observed in <10% of progeny.
Stp Observed in >=10% of progeny.
Phenotype  Aberrant AB or P1 spindle orientation
Aberrant cell-cell contacts at the four-cell stage
Cytokinesis defect
Ectopic cleavage furrows
EMS fails to extend anteriorly
Exaggerated pseudocleavage
Excessive blebbing
Loose mitotic furrow
Multiple nuclei in 1, 2, or 4-cell embryos
P0 spindle does not rotate
Pronuclear breakdown asynchronous
Movies/Stacks/Images  /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif Help
PF:GL1_3A1 Experiment Details Ace View
Laboratory  KK
RNAi Reagent & Type  sjj_F49E11.1 (PCR_reagent)
Remark  PCR product contains flanking T7 promoter sequences
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
Delivery Method  Injection
Date  2002-11-19
Gene Plot of F49E11.1 Browse using GBrowse
Gene Graph